Isolation and Characterization of New Bacteriophages against Staphylococcal Clinical Isolates from Diabetic Foot Ulcers

Lucile Plumet (1) , Madjid Morsli (2, 3) , Nour Ahmad-Mansour (1) , Fernando Clavijo-Coppens (4) , Laurence Berry (5) , Albert Sotto (1) , Jean-Philippe Lavigne (1) , Denis Costechareyre (4) , Virginie Molle (1)

1 VBIC - Virulence Bactérienne et Infections Chroniques
2 MEPHI - Microbes évolution phylogénie et infections
3 IHU Marseille - Institut Hospitalier Universitaire Méditerranée Infection
4 Greenphage
5 LPHI - Laboratory of Pathogen and Host Immunity [Montpellier]

Staphylococcus sp. is the most common bacterial genus in infections related to diabetic foot ulcers (DFUs). The emergence of multidrug-resistant bacteria places a serious burden on public health systems. Phage therapy is an alternative treatment to antibiotics, overcoming the issue of antibiotic resistance. In this study, six phages (SAVM01 to SAVM06) were isolated from effluents and were used against a panel of staphylococcal clinical samples isolated from DFUs. A genomic analysis revealed that the phages belonged to the Herelleviridae family, with sequences similar to those of the Kayvirus genus. No lysogeny-associated genes, known virulence or drug resistance genes were identified in the phage genomes. The phages displayed a strong lytic and antibiofilm activity against DFU clinical isolates, as well as against opportunistic pathogenic coagulase-negative staphylococci. The results presented here suggest that these phages could be effective biocontrol agents against staphylococcal clinical isolates from DFUs.

Keywords: Sotto; A.; Lavigne; J.-P.; Costechareyre; D.; Molle; V. Isolation and Characterization of New Bacteriophages against Staphylococcal Clinical Isolates from bacteriophages; biofilm; diabetic foot ulcer; Kayvirus; Staphylococcus sp; Plumet; L. Morsli; M. Ahmad-Mansour; N. Clavijo-Coppens; F. Berry; L. Sotto; A. Lavigne; J.-P. Costechareyre; D. Molle; V. Isolation and Characterization of New Bacteriophages against Staphylococcal Clinical Isolates from bacteriophages biofilm diabetic foot ulcer Kayvirus Staphylococcus sp; L.; Morsli; M.; Ahmad-Mansour; N.; Clavijo-Coppens; F.; Berry

Domains: Life Sciences [q-bio]

Lucile Plumet, Madjid Morsli, Nour Ahmad-Mansour, Fernando Clavijo-Coppens, Laurence Berry, et al.. Isolation and Characterization of New Bacteriophages against Staphylococcal Clinical Isolates from Diabetic Foot Ulcers. Viruses, 2023, 15 (12), pp.2287. ⟨10.3390/v15122287⟩. ⟨hal-04360134⟩