Characterization of Benchtop-Fabricated Arrays of Nanowrinkled Surface Electrodes as a Nitric Oxide Electrochemical Sensor

Cindy Peto-Gutiérrez (1) , Genaro Vázquez-Victorio (1) , Mathieu Hautefeuille (2)

1 UNAM - Universidad Nacional Autónoma de México = National Autonomous University of Mexico
2 LBD - Laboratoire de Biologie du Développement [IBPS]

In this work, we present an accessible benchtop fabrication technique to obtain a planar array of gold nanowrinkled surface electrodes (ANSE) for the construction of electrochemical cells, specifically to monitor soluble biomarkers of interest in cell culture environments. We present a complete characterization of the array and its response as an electrochemical cell. To validate our sensor, we evaluated the device sensitivity to detect nitric oxide (NO), an important molecule produced by endothelial cells as a response to environmental signals such as mechanics and growth factors. While testing measurements of nitric oxide in aqueous solutions with isotonic salt concentrations, we evidenced the influence of the environmental conditions for such electrochemical measurements, showing that the aqueous medium, usually not accounted for, significantly impacts the outcome. Finally, we present the application of the electrochemical sensor for the detection of nitric oxide released from stimulated endothelial cells as a proof of concept.

Mots clés: nanowrinkled surface electrodes extracellular media sensing nitric oxide; nanowrinkled surface electrodes; extracellular media sensing; nitric oxide

Domaines: Sciences de l'ingénieur [physics]

Cindy Peto-Gutiérrez, Genaro Vázquez-Victorio, Mathieu Hautefeuille. Characterization of Benchtop-Fabricated Arrays of Nanowrinkled Surface Electrodes as a Nitric Oxide Electrochemical Sensor. Biosensors, 2023, 13, ⟨10.3390/bios13080794⟩. ⟨hal-04194196⟩