Co-Immobilization of D-Amino Acid Oxidase, Catalase, and Transketolase for One-Pot, Two-Step Synthesis of L-Erythrulose

Daria Świętochowska (1) , Aleksandra Łochowicz (1) , Nazim Ocal (2) , Loredano Pollegioni (3) , Franck Charmantray (2) , Laurence Hecquet (2) , Katarzyna Szymańska (4)

1 Department of Organic Chemistry, Bioorganic Chemistry and Biotechnology, Silesian University of Technology
2 ICCF - Institut de Chimie de Clermont-Ferrand
3 Department of Biotechnology and Life Sciences
4 Department of Chemical Engineering and Process Design, Silesian University of Technology

Here, we present an immobilized enzyme cascade in a basket-type reactor allowing a one-pot, two-step enzymatic synthesis of L-erythrulose from D-serine and glycolaldehyde. Three enzymes, D-amino acid oxidase from Rhodotorula gracilis (DAAORg), catalase from bovine liver (CAT), and transketolase from Geobacillus stearothermophilus (TKgst) were covalently immobilized on silica monolithic pellets, characterized by an open structure of interconnected macropores and a specific surface area of up to 300 m2/g. Three strategies were considered: (i) separate immobilization of enzymes on silica supports ([DAAO][CAT][TK]), (ii) co-immobilization of two of the three enzymes followed by the third ([DAAO+CAT][TK]), and (iii) co-immobilization of all three enzymes ([DAAO+CAT+TK]). The highest L-erythrulose concentrations were observed for the co-immobilization protocols (ii) and (iii) (30.7 mM and 29.1 mM, respectively). The reusability study showed that the best combination was [DAAO + CAT][TK], which led to the same level of L-erythrulose formation after two reuse cycles. The described process paves the way for the effective synthesis of a wide range of α-hydroxyketones from D-serine and suitable aldehydes.

Mots clés: co-immobilization; one-pot; two-step cascade; monolithic silica pellets; modular production system of L

Domaines: Chimie

Daria Świętochowska, Aleksandra Łochowicz, Nazim Ocal, Loredano Pollegioni, Franck Charmantray, et al.. Co-Immobilization of D-Amino Acid Oxidase, Catalase, and Transketolase for One-Pot, Two-Step Synthesis of L-Erythrulose. Catalysts, 2023, 13 (1), pp.95. ⟨10.3390/catal13010095⟩. ⟨hal-03922760⟩