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Theses

Activité transcriptionnelle de la protéine ribosomique uL11

Abstract : In drosophila, the ribosomal protein uL11 is involved in translation and regulates transcription. Indeed, uL11 trimethylated on lysine 3 directly interacts with the enhancer of Trithorax and Polycomb Corto and co-localises with it on many sites on polytene chromosomes. Furthermore, RNA-seq analyses of larval wing imaginal discs over-expressing uL11 or the chromodomain of Corto show that many ribosomal biogenesis and ribosomal protein genes are up-regulated. Three mutants of uL11K3 were obtained by CRISPR-Cas9 (uL11K3A, uL11K3Y et uL11ΔK3). Puromycine incorporation showed that only uL11K3A has a decreased translation in agreement with its dominant Minute phenotypes (which include high lethality, longer development, shorter bristles). Co-immunoprecipitation experiments show that uL11K3A and uL11K3Y do not interact with the chromodomain of Corto. Whereas, surprisingly, uL11ΔK3 still interacted with it. RNA-seq analyses of the wing imaginal disc transcriptomes of uL11K3A and uL11K3Y show that few genes are deregulated (414 genes for the former, 84 genes for the latter). As in Saccharomyces cerevisiae, uL11 controls transcription of genes of the PHO pathway in low-phosphate conditions. We next tested whether the uL11 mutants were more affected in restrictive diet conditions. We focused on the uL11K3Y mutants which are unaffected in normal conditions. In a restrictive diet, developmental time of uL11K3Y mutants is longer than the wild-type one, and their wings are smaller. Thus, in drosophila as S. cerevisiae, the transcriptional activity of uL11 might be important in poor environmental conditions.
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https://tel.archives-ouvertes.fr/tel-03465133
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Submitted on : Friday, December 3, 2021 - 4:35:07 PM
Last modification on : Tuesday, January 4, 2022 - 5:49:13 AM

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GRUNCHEC_Heloise_2021.pdf
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  • HAL Id : tel-03465133, version 1

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Heloïse Grunchec. Activité transcriptionnelle de la protéine ribosomique uL11. Biologie du développement. Sorbonne Université, 2021. Français. ⟨NNT : 2021SORUS195⟩. ⟨tel-03465133⟩

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