The molecular landscape of glioma in patients with Neurofibromatosis 1

Fulvio d'Angelo , Michele Ceccarelli (1) , Luciano Garofano (2, 3) , Jing Zhang , Véronique Frattini , Francesca Caruso , Genevieve Lewis (2) , Kristin Alfaro , Luc Bauchet (4, 5) , Giulia Berzero , David Cachia (6) , Mario Cangiano , Laurent Capelle (7) , John de Groot (8) , Francesco Dimeco (9, 10) , François Ducray (11) , Walid Farah (12) , Gaetano Finocchiaro (9) , Stéphane Goutagny (13) , Carlos Kamiya-Matsuoka (8) , Cinzia Lavarino , Hugues Loiseau , Véronique Lorgis , Carlo Marras , Ian Mccutcheon , Do-Hyun Nam , Susanna Ronchi , Veronica Saletti , Romuald Seizeur , John Slopis , Mariona Suñol , Fanny Vandenbos , Pascale Varlet , Dominique Vidaud , Colin Watts , Viviane Tabar , David Reuss , Seung-Ki Kim , David Meyronet , Karima Mokhtari , Hector Salvador , Krishna Bhat , Marica Eoli , Marc Sanson , Anna Lasorella , Antonio Iavarone , Fulvio D’angelo

1 Department of Science and Technology
2 CUMC - Columbia University Medical Center
3 University of Sannio [Benevento]
4 INM - Institut des Neurosciences de Montpellier
5 CHRU Montpellier - Centre Hospitalier Régional Universitaire [Montpellier]
6 MUSC - Medical University of South Carolina [Charleston]
7 Service de Neurochirurgie [CHU Pitié-Salpêtrière]
8 The University of Texas M.D. Anderson Cancer Center [Houston]
9 Fondazione IRCCS Istituto Neurologico "Carlo Besta"
10 UNIMI - Università degli Studi di Milano = University of Milan
11 Centre de Référence Maladie Rare "Syndromes neurologiques Paranéoplasiques"
12 Service de neurochirurgie [CHU de Dijon]
13 Service de neurochirurgie

Neurofibromatosis type 1 (NF1) is a common tumor predisposition syndrome in which glioma is one of the prevalent tumors. Gliomagenesis in NF1 results in a heterogeneous spectrum of low- to high-grade neoplasms occurring during the entire lifespan of patients. The pattern of genetic and epigenetic alterations of glioma that develops in NF1 patients and the similarities with sporadic glioma remain unknown. Here, we present the molecular landscape of low- and high-grade gliomas in patients affected by NF1 (NF1-glioma). We found that the predisposing germline mutation of the NF1 gene was frequently converted to homozygosity and the somatic mutational load of NF1-glioma was influenced by age and grade. High-grade tumors harbored genetic alterations of TP53 and CDKN2A, frequent mutations of ATRX associated with Alternative Lengthening of Telomere, and were enriched in genetic alterations of transcription/chromatin regulation and PI3 kinase pathways. Low-grade tumors exhibited fewer mutations that were over-represented in genes of the MAP kinase pathway. Approximately 50% of low-grade NF1-gliomas displayed an immune signature, T lymphocyte infiltrates, and increased neo-antigen load. DNA methylation assigned NF1-glioma to LGm6, a poorly defined Isocitrate Dehydrogenase 1 wild-type subgroup enriched with ATRX mutations. Thus, the profiling of NF1-glioma defined a distinct landscape that recapitulates a subset of sporadic tumors.

Domains: Life Sciences [q-bio]; Human health and pathology; Surgery; Life Sciences [q-bio]; Cancer

Fulvio d'Angelo, Michele Ceccarelli, Luciano Garofano, Jing Zhang, Véronique Frattini, et al.. The molecular landscape of glioma in patients with Neurofibromatosis 1. Nature Medicine, 2019, 25 (1), pp.176-187. ⟨10.1038/s41591-018-0263-8⟩. ⟨hal-03905472⟩