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How to make and trap a pseudo-vesicle with a micropipette

Abstract : We present a simple method to produce giant lipid pseudo-vesicles (vesicles with an oily cap on the top), trapped in an agarose gel. The method can be implemented using only a regular micropipette and relies on the formation of a water/oil/water double droplet in liquid agarose. We characterize the produced vesicle with fluorescence imaging and establish the presence and integrity of the lipid bilayer by the successful insertion of {\alpha}-Hemolysin transmembrane proteins. Finally, we show that the vesicle can be easily mechanically deformed, non-intrusively, by indenting the surface of the gel.
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Preprints, Working Papers, ... (Preprint)
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https://hal-cnrs.archives-ouvertes.fr/hal-03865909
Contributor : Alexis Prevost Connect in order to contact the contributor
Submitted on : Tuesday, November 22, 2022 - 3:07:30 PM
Last modification on : Wednesday, November 23, 2022 - 4:11:21 AM

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  • HAL Id : hal-03865909, version 1
  • ARXIV : 2207.06402

Citation

Pierre Tapie, Alexis M. Prevost, Lorraine Montel, Léa-Laetitia Pontani, Elie Wandersman. How to make and trap a pseudo-vesicle with a micropipette. {date}. ⟨hal-03865909⟩

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