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Nuclear-enriched protein phosphatase 4 ensures outer kinetochore assembly prior to nuclear dissolution

Abstract : ABSTRACT A landmark event in the transition from interphase to mitosis in metazoans is nuclear envelope breakdown (NEBD). Many events important for mitosis occur prior to NEBD, including condensation of replicated chromosomes and assembly of kinetochores to rapidly engage spindle microtubules. Here we show that nuclear-enriched protein phosphatase 4 (PP4) ensures robust assembly of the microtubule-coupling outer kinetochore prior to NEBD. In the absence of PP4, chromosomes exhibit extended monopolar orientation after NEBD and subsequently mis-segregate. A secondary consequence of diminished outer kinetochore assembly is defective sister chromatid resolution. After NEBD, a cytoplasmic activity compensates for PP4 loss, leading to outer kinetochore assembly and recovery of chromosomes from monopolar orientation to significant biorientation. The Ndc80-Ska microtubule-binding module of the outer kinetochore is required for this recovery. PP4 associates with the inner kinetochore protein CENP-C; however, disrupting the PP4–CENP-C interaction does not perturb chromosome segregation. These results establish that PP4-dependent outer kinetochore assembly prior to NEBD is critical for timely and proper engagement of chromosomes with spindle microtubules.
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Preprints, Working Papers, ...
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Contributor : Julien DUMONT Connect in order to contact the contributor
Submitted on : Tuesday, November 22, 2022 - 9:44:43 AM
Last modification on : Tuesday, November 22, 2022 - 9:45:07 AM

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Helder Rocha, Patrícia Simões, Jacqueline Budrewicz, Pablo Lara-Gonzalez, Ana Xavier Carvalho, et al.. Nuclear-enriched protein phosphatase 4 ensures outer kinetochore assembly prior to nuclear dissolution. {date}. ⟨hal-03864952⟩



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