Skip to Main content Skip to Navigation
New interface
Preprints, Working Papers, ... (Preprint)

Annexin A1 is a polarity cue that directs planar mitotic spindle orientation during mammalian epithelial morphogenesis

Abstract : Abstract Oriented cell divisions are critical for the formation and maintenance of structured epithelia. Proper mitotic spindle orientation relies on polarised anchoring of force generators to the cell cortex by the evolutionarily conserved G αi -LGN-NuMA complex. However, the polarity cues that control cortical patterning of this ternary complex remain largely unknown in mammalian epithelia. Here we identify the membrane-associated protein Annexin A1 (ANXA1) as a novel interactor of LGN in mammary epithelial cells. ANXA1 acts independently of G αi to instruct the accumulation of LGN and NuMA at the lateral cortex to ensure cortical anchoring of Dynein-Dynactin and astral microtubules and thereby planar alignment of the mitotic spindle. Loss of ANXA1 randomises mitotic spindle orientation, which in turn disrupts epithelial architecture and luminogenesis in three-dimensional cultures of primary mammary epithelial cells. Our findings establish ANXA1 as an upstream cortical cue that regulates LGN to direct planar cell divisions during mammalian epithelial morphogenesis.
Document type :
Preprints, Working Papers, ... (Preprint)
Complete list of metadata

https://hal-cnrs.archives-ouvertes.fr/hal-03862421
Contributor : Xavier Morin Connect in order to contact the contributor
Submitted on : Monday, November 21, 2022 - 10:13:32 AM
Last modification on : Friday, November 25, 2022 - 12:05:19 PM

Links full text

Identifiers

Collections

Citation

Maria Fankhaenel, Farahnaz Sadat Golestan Hashemi, Manal Mosa Hosawi, Larissa Mourao, Paul Skipp, et al.. Annexin A1 is a polarity cue that directs planar mitotic spindle orientation during mammalian epithelial morphogenesis. 2022. ⟨hal-03862421⟩

Share

Metrics

Record views

0