Inflammatory profile of convalescent plasma to treat COVID: Impact of amotosalen/UVA pathogen reduction technology - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Frontiers in Immunology Année : 2022

Inflammatory profile of convalescent plasma to treat COVID: Impact of amotosalen/UVA pathogen reduction technology

Fabrice Cognasse
  • Fonction : Auteur
Hind Hamzeh-Cognasse
  • Fonction : Auteur
Anne-Claire Duchez
  • Fonction : Auteur
Natalia Shurko
  • Fonction : Auteur
Marie-Ange Eyraud
  • Fonction : Auteur
Charles-Antoine Arthaud
  • Fonction : Auteur
Amélie Prier
  • Fonction : Auteur
Marco Heestermans
  • Fonction : Auteur
Olivier Hequet
  • Fonction : Auteur
Brigitte Bonneaudeau
  • Fonction : Auteur
Sandrine Rochette-Eribon
  • Fonction : Auteur
Françoise Teyssier
  • Fonction : Auteur
Valérie Barlet-Excoffier
  • Fonction : Auteur
Patricia Chavarin
  • Fonction : Auteur
Dominique Legrand
  • Fonction : Auteur
Pascale Richard
  • Fonction : Auteur
Pascal Morel
  • Fonction : Auteur
Pierre Tiberghien
  • Fonction : Auteur

Résumé

Blood products in therapeutic transfusion are now commonly acknowledged to contain biologically active constituents during the processes of preparation. In the midst of a worldwide COVID-19 pandemic, preliminary evidence suggests that convalescent plasma may lessen the severity of COVID-19 if administered early in the disease, particularly in patients with profound B-cell lymphopenia and prolonged COVID-19 symptoms. This study examined the influence of photochemical Pathogen Reduction Treatment (PRT) using amotosalen‐HCl and UVA light in comparison with untreated control convalescent plasma (n= 72 – paired samples) - cFFP, regarding soluble inflammatory factors: sCD40L, IFN-alpha, IFN-beta, IFN-gamma, IL-1 beta, IL-6, IL-8, IL-10, IL-18, TNF-alpha and ex-vivo inflammatory bioactivity on endothelial cells. We didn’t observe significant modulation of the majority of inflammatory soluble factors (8 of 10 molecules tested) pre- or post-PRT. We noted that IL-8 concentrations were significantly decreased in cFFP with PRT, whereas the IL-18 concentration was increased by PRT. In contrast, endothelial cell release of IL-6 was similar whether cFFP was pre-treated with or without PRT. Expression of CD54 and CD31 in the presence of cFFP were similar to control levels, and both were significant decreased in when cFFP had been pre-treated by PRT. It will be interesting to continue investigations of IL-18 and IL-8, and the physiopathological effect of PRT- treated convalescent plasma and in clinical trials. But overall, it appears that cFFP post-PRT were not excessively pro-inflammatory. Further research, including a careful clinical evaluation of CCP-treated patients, will be required to thoroughly define the clinical relevance of these findings.

Dates et versions

hal-03855611 , version 1 (16-11-2022)

Identifiants

Citer

Fabrice Cognasse, Hind Hamzeh-Cognasse, Anne-Claire Duchez, Natalia Shurko, Marie-Ange Eyraud, et al.. Inflammatory profile of convalescent plasma to treat COVID: Impact of amotosalen/UVA pathogen reduction technology. Frontiers in Immunology, 2022, 13, ⟨10.3389/fimmu.2022.1034379⟩. ⟨hal-03855611⟩

Collections

CEA CNRS
8 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More