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De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals

Guillaume Tetreau 1 Michael Sawaya 2 Elke de Zitter 1 Elena Andreeva 3 Anne-Sophie Banneville 1 Natalie Schibrowsky Nicolas Coquelle 1 Aaron Brewster Marie Luise Grünbein 4 Gabriela Nass Kovacs Mark Hunter Marco Kloos 5 Raymond Sierra 6 Giorgio Schiro 1 Pei Qiao Myriam Stricker Dennis Bideshi 7 Iris Young 8 Ninon Zala 3 Sylvain Engilberge 9 Alexander Gorel Luca Signor 1 Jean-Marie Teulon 1 Mario Hilpert Lutz Foucar Johan Bielecki Richard Bean Raphael de Wijn Tokushi Sato Henry Kirkwood Romain Letrun Alexander Batyuk Irina Snigireva Daphna Fenel 1 Robin Schubert Ethan Canfield Mario Alba Frédéric Laporte 10 Laurence Després 10 Maria Bacia 1 Amandine Roux 11 Christian Chapelle François Riobé 11 Olivier Maury 11 Wai Li Ling 1 Sébastien Boutet 12 Adrian Mancuso Irina Gutsche 1 Eric Girard 1 Thomas Barends Jean-Luc Pellequer 1 Hyun-Woo Park Arthur Laganowsky Jose Rodriguez Manfred Burghammer Robert Shoeman R. Bruce Doak Martin Weik 1 Nicholas Sauter Brian Federici Duilio Cascio Ilme Schlichting Jacques-Philippe Colletier 1 
Abstract : Cry11Aa and Cry11Ba are the two most potent toxins produced by mosquitocidal Bacillus thuringiensis subsp. israelensis and jegathesan , respectively. The toxins naturally crystallize within the host; however, the crystals are too small for structure determination at synchrotron sources. Therefore, we applied serial femtosecond crystallography at X-ray free electron lasers to in vivo-grown nanocrystals of these toxins. The structure of Cry11Aa was determined de novo using the single-wavelength anomalous dispersion method, which in turn enabled the determination of the Cry11Ba structure by molecular replacement. The two structures reveal a new pattern for in vivo crystallization of Cry toxins, whereby each of their three domains packs with a symmetrically identical domain, and a cleavable crystal packing motif is located within the protoxin rather than at the termini. The diversity of in vivo crystallization patterns suggests explanations for their varied levels of toxicity and rational approaches to improve these toxins for mosquito control.
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https://hal-cnrs.archives-ouvertes.fr/hal-03847393
Contributor : Jacques-Philippe Colletier Connect in order to contact the contributor
Submitted on : Thursday, November 24, 2022 - 10:43:02 AM
Last modification on : Tuesday, November 29, 2022 - 12:06:07 PM

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Guillaume Tetreau, Michael Sawaya, Elke de Zitter, Elena Andreeva, Anne-Sophie Banneville, et al.. De novo determination of mosquitocidal Cry11Aa and Cry11Ba structures from naturally-occurring nanocrystals. Nature Communications, 2022, 13 (1), pp.4376. ⟨10.1038/s41467-022-31746-x⟩. ⟨hal-03847393⟩

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