Skip to Main content Skip to Navigation
New interface
Journal articles

Protocol to measure cleavage efficiency of the meiotic cohesin subunit Rec8 by separase in mouse oocytes using a biosensor

Abstract : Here we describe a biosensor to assess meiotic cohesin cleavage by live imaging in mouse oocytes. The biosensor is targeted to chromosomes and consists of two fluorophores flanking a fragment of Rec8 containing Separase cleavage sites. Cleavage of Rec8 leads to dissociation of one of the fluorophores from chromosomes which is used to estimate cleavage efficiency. We detail the use of this biosensor in mouse oocytes completing the first meiotic division in presence or absence of AuroraB/C inhibitor. For details on the application of this protocol, please refer to (Nikalayevich et al., 2022).
Complete list of metadata

https://hal-cnrs.archives-ouvertes.fr/hal-03809570
Contributor : Katja Wassmann Connect in order to contact the contributor
Submitted on : Wednesday, October 12, 2022 - 11:42:01 AM
Last modification on : Thursday, October 27, 2022 - 3:58:36 AM

File

CellPress_STARProtocols for Ha...
Files produced by the author(s)

Identifiers

Citation

Elvira Nikalayevich, Katja Wassmann. Protocol to measure cleavage efficiency of the meiotic cohesin subunit Rec8 by separase in mouse oocytes using a biosensor. STAR Protocols, In press, 3 (4), pp.101714. ⟨10.1016/j.xpro.2022.101714⟩. ⟨hal-03809570⟩

Share

Metrics

Record views

0

Files downloads

0