Skip to Main content Skip to Navigation
New interface
Journal articles

New insights into detecting alizarin from autofluorescence in marked glass eels

Abstract : Alizarin detection in fish fins is extensively employed because it is easy to use. However, in eels, the eelGFP fluorescent protein may impede the detection of the fluorescent markers in the eel tissues. The study tests the effectiveness of three of the most up-to-date alizarin-detecting technologies on the living body and fins of European glass eels ( Anguilla anguilla L.). The findings demonstrated that the control group had a high autofluorescence at alizarin and eelGFP maxima bands. With fluorescence reflectance imaging (FRI), the eel living body autofluorescence impeded the detection of the marked eels. In contrast with experimental excitation-emission-matrix (EEM) fluorescence analyses, 99% of the marked eels were correctly assigned to their group from fluorescence analyses of their fin cellular contents. With epifluorometry (EPI), 100% of the marked eels were detected with the caudal fin tips when excited at 450–490 nm wavelengths due to a weaker autofluorescence signal. EEM and FRI assays unveiled an average fluorescence quenching 60% and 44% of the marked group respectively, in the alizarin and eelGFP maxima bands. The fluorescence quenching observed is discussed. Results will benefit experimental design by examining autofluorescence effects on mark detection and the development of non-invasive detection methods in this critically endangered species.
Document type :
Journal articles
Complete list of metadata

https://hal-cnrs.archives-ouvertes.fr/hal-03798587
Contributor : Edith PARLANTI Connect in order to contact the contributor
Submitted on : Wednesday, October 5, 2022 - 12:31:30 PM
Last modification on : Friday, November 25, 2022 - 10:03:34 AM

File

Gaillard22.pdf
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution 4.0 International License

Identifiers

Collections

Citation

Mélanie Gaillard, Edith Parlanti, Mahaut Sourzac, Franck Couillaud, Coralie Genevois, et al.. New insights into detecting alizarin from autofluorescence in marked glass eels. Scientific Reports, 2022, 12 (1), pp.15985. ⟨10.1038/s41598-022-18440-0⟩. ⟨hal-03798587⟩

Share

Metrics

Record views

23

Files downloads

1