Structure-Function analysis of Lactiplantibacillus plantarum DltE reveals D-alanylated lipoteichoic acids as direct symbiotic cues supporting Drosophila juvenile growth

Nikos Nikolopoulos , Renata C Matos (1) , Stephanie Ravaud , Pascal Courtin , Houssam Akherraz , Simon Palussiere , Virginie Gueguen-Chaignon , Marie Salomon-Mallet , Alain Guillot , Yann Guerardel , Marie-Pierre Chapot-Chartier , Christophe Grangeasse , Francois Leulier

1 IGFL - Institut de Génomique Fonctionnelle de Lyon

Metazoans establish mutually beneficial interactions with their resident microorganisms. However, our understanding of the microbial cues contributing to host physiology remains elusive. Previously, we identified a bacterial machinery encoded by the dlt operon involved in Drosophila melanogaster's juvenile growth promotion by Lactiplantibacillus plantarum. Here, using crystallography combined with biochemical and cellular approaches, we investigate the physiological role of an uncharacterized protein (DltE) encoded by this operon. We show that LTAs but not WTAs are D-alanylated in Lactiplantibacillus plantarum NC8 cell envelope and demonstrate that DltE is a D-Ala carboxyesterase removing D-Ala from LTA. Using the mutualistic association of L. plantarum NC8 and Drosophila melanogaster as a symbiosis model, we establish that D-Ala-LTAs are direct symbiotic cues supporting intestinal peptidase expression and juvenile growth in Drosophila. Our results pave the way to probing the contribution of D-Ala-LTA to host physiology in other symbiotic models.

Domaines: Sciences du Vivant [q-bio]

Nikos Nikolopoulos, Renata C Matos, Stephanie Ravaud, Pascal Courtin, Houssam Akherraz, et al.. Structure-Function analysis of Lactiplantibacillus plantarum DltE reveals D-alanylated lipoteichoic acids as direct symbiotic cues supporting Drosophila juvenile growth. 2022. ⟨hal-03778085⟩