Structure-Function analysis of Lactiplantibacillus plantarum DltE reveals D-alanylated lipoteichoic acids as direct symbiotic cues supporting Drosophila juvenile growth - Archive ouverte HAL Access content directly
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Structure-Function analysis of Lactiplantibacillus plantarum DltE reveals D-alanylated lipoteichoic acids as direct symbiotic cues supporting Drosophila juvenile growth

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Nikos Nikolopoulos
  • Function : Author
Renata C Matos
Stephanie Ravaud
  • Function : Author
Pascal Courtin
  • Function : Author
Houssam Akherraz
  • Function : Author
Simon Palussiere
  • Function : Author
Virginie Gueguen-Chaignon
  • Function : Author
Marie Salomon-Mallet
  • Function : Author
Alain Guillot
  • Function : Author
Yann Guerardel
  • Function : Author
Marie-Pierre Chapot-Chartier
Christophe Grangeasse
  • Function : Author
  • PersonId : 1109143
Francois Leulier

Abstract

Metazoans establish mutually beneficial interactions with their resident microorganisms. However, our understanding of the microbial cues contributing to host physiology remains elusive. Previously, we identified a bacterial machinery encoded by the dlt operon involved in Drosophila melanogaster's juvenile growth promotion by Lactiplantibacillus plantarum. Here, using crystallography combined with biochemical and cellular approaches, we investigate the physiological role of an uncharacterized protein (DltE) encoded by this operon. We show that LTAs but not WTAs are D-alanylated in Lactiplantibacillus plantarum NC8 cell envelope and demonstrate that DltE is a D-Ala carboxyesterase removing D-Ala from LTA. Using the mutualistic association of L. plantarum NC8 and Drosophila melanogaster as a symbiosis model, we establish that D-Ala-LTAs are direct symbiotic cues supporting intestinal peptidase expression and juvenile growth in Drosophila. Our results pave the way to probing the contribution of D-Ala-LTA to host physiology in other symbiotic models.
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hal-03778085 , version 1 (15-09-2022)

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Nikos Nikolopoulos, Renata C Matos, Stephanie Ravaud, Pascal Courtin, Houssam Akherraz, et al.. Structure-Function analysis of Lactiplantibacillus plantarum DltE reveals D-alanylated lipoteichoic acids as direct symbiotic cues supporting Drosophila juvenile growth. 2022. ⟨hal-03778085⟩
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