Light-inducible T cell engagers trigger, tune and shape the activation of primary T cells - CNRS - Centre national de la recherche scientifique Accéder directement au contenu
Article Dans Une Revue Proceedings of the National Academy of Sciences of the United States of America Année : 2023

Light-inducible T cell engagers trigger, tune and shape the activation of primary T cells

Résumé

Cells perceive overtime complex sequences of receptor stimulation that they integrate to mount an appropriate response. Yet, the influence of signal dynamics on cell responses has been poorly characterized due to technical limitations. Here, we present a generalizable approach to control receptor stimulation on unmodified primary cells. Indeed, for applications on primary murine T cells, we have engineered the LiTe system, a new recombinant optogenetics-based Light-inducible T cell engager which allows tunable and reversible spatiotemporal control of the T Cell Receptor (TCR) stimulation. We also provided in vitro evidence that this system enables efficient T cell activation with light, leading to cytokine secretion or tumor cell killing. Using specific time-gated stimulations, we have been able to orient the outcome of the activation of T cells. Overall, the LiTe system constitutes a versatile ON/OFF molecular switch allowing to decipher the cellular response to stimulation dynamics. Its original control over T cell activation opens new avenues for future precision cancer immunotherapy.
Fichier principal
Vignette du fichier
jaeger-et-al-2023-light-inducible-t-cell-engagers-trigger-tune-and-shape-the-activation-of-primary-t-cells.pdf (992.16 Ko) Télécharger le fichier
Origine : Publication financée par une institution
Licence : CC BY - Paternité

Dates et versions

hal-03775201 , version 1 (12-09-2022)
hal-03775201 , version 2 (15-11-2023)

Identifiants

Citer

Morgane Jaeger, Amandine Anastasio, Léa Chamy, Sophie Brustlein, Renaud Vincentelli, et al.. Light-inducible T cell engagers trigger, tune and shape the activation of primary T cells. Proceedings of the National Academy of Sciences of the United States of America, 2023, 120 (39), pp.e2302500120. ⟨10.1073/pnas.2302500120⟩. ⟨hal-03775201v2⟩
56 Consultations
76 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More