Biodegradable Nanoparticles Loaded with Levodopa and Curcumin for Treatment of Parkinson’s Disease - Archive ouverte HAL Access content directly
Journal Articles Molecules Year : 2022

Biodegradable Nanoparticles Loaded with Levodopa and Curcumin for Treatment of Parkinson’s Disease

, , , , , , , , , , , , (1) , ,
1
Bassam Felipe Mogharbel
Marco André Cardoso
  • Function : Author
Ana Carolina Irioda
  • Function : Author
Priscila Elias Ferreira Stricker
Robson Camilotti Slompo
  • Function : Author
Julia Maurer Appel
  • Function : Author
Nathalia Barth de Oliveira
Maiara Carolina Perussolo
Claudia Sayuri Saçaki
  • Function : Author
Nadia Nascimento da Rosa
  • Function : Author
Dilcele Silva Moreira Dziedzic
  • Function : Author
Christophe Travelet
Redouane Borsali
  • Function : Author
Katherine Athayde Teixeira de Carvalho

Abstract

Background: Parkinson’s disease (PD) is the second most common age-related neurodegenerative disorder. Levodopa (L-DOPA) remains the gold-standard drug available for treating PD. Curcumin has many pharmacological activities, including antioxidant, anti-inflammatory, antimicrobial, anti-amyloid, and antitumor properties. Copolymers composed of Poly (ethylene oxide) (PEO) and biodegradable polyesters such as Poly (ε-caprolactone) (PCL) can self-assemble into nanoparticles (NPs). This study describes the development of NH2–PEO–PCL diblock copolymer positively charged and modified by adding glutathione (GSH) on the outer surface, resulting in a synergistic delivery of L-DOPA curcumin that would be able to pass the blood–brain barrier. Methods: The NH2–PEO–PCL NPs suspensions were prepared by using a nanoprecipitation and solvent displacement method and coated with GSH. NPs were submitted to characterization assays. In order to ensure the bioavailability, Vero and PC12 cells were treated with various concentrations of the loaded and unloaded NPs to observe cytotoxicity. Results: NPs have successfully loaded L-DOPA and curcumin and were stable after freeze-drying, indicating advancing into in vitro toxicity testing. Vero and PC12 cells that were treated up to 72 h with various concentrations of L-DOPA and curcumin-loaded NP maintained high viability percentage, indicating that the NPs are biocompatible. Conclusions: NPs consisting of NH2–PEO–PCL were characterized as potential formulations for brain delivery of L-DOPA and curcumin. The results also indicate that the developed biodegradable nanomicelles that were blood compatible presented low cytotoxicity.

Dates and versions

hal-03755460 , version 1 (22-08-2022)

Identifiers

Cite

Bassam Felipe Mogharbel, Marco André Cardoso, Ana Carolina Irioda, Priscila Elias Ferreira Stricker, Robson Camilotti Slompo, et al.. Biodegradable Nanoparticles Loaded with Levodopa and Curcumin for Treatment of Parkinson’s Disease. Molecules, 2022, 27 (9), pp.2811. ⟨10.3390/molecules27092811⟩. ⟨hal-03755460⟩
5 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More