Lung tissue biomechanics imaged with synchrotron phase contrast microtomography in live rats - Archive ouverte HAL Access content directly
Journal Articles Scientific Reports Year : 2022

Lung tissue biomechanics imaged with synchrotron phase contrast microtomography in live rats

(1) , (2) , (3) , (4) , (1) , (5) , (6)
1
2
3
4
5
6

Abstract

The magnitude and distribution of strain imposed on the peripheral airspaces by mechanical ventilation at the microscopic level and the consequent deformations are unknown despite their importance for understanding the mechanisms occurring at the onset of ventilator-induced lung injury. Here a 4-Dimensional (3D + time) image acquisition and processing technique is developed to assess pulmonary acinar biomechanics at microscopic resolution. Synchrotron radiation phase contrast CT with an isotropic voxel size of 6 mu m(3) is applied in live anesthetized rats under controlled mechanical ventilation. Video animations of regional acinar and vascular strain are acquired in vivo. Maps of strain distribution due to positive-pressure breaths and cardiovascular activity in lung acini and blood vessels are derived based on CT images. Regional strain within the lung peripheral airspaces takes average values of 0.09 +/- 0.02. Fitting the expression S = kV(n), to the changes in peripheral airspace area (S) and volume (V) during a positive pressure breath yields an exponent n = 0.82 +/- 0.03, suggesting predominant alveolar expansion rather than ductal expansion or alveolar recruitment. We conclude that this methodology can be used to assess acinar conformational changes during positive pressure breaths in intact peripheral lung airspaces.
Fichier principal
Vignette du fichier
Sci_Reports_12_5056.pdf (3.57 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive

Dates and versions

hal-03722030 , version 1 (13-07-2022)

Identifiers

Cite

Jose-Luis Cercos-Pita, Luca Fardin, Hugo Leclerc, Bertrand Maury, Gaetano Perchiazzi, et al.. Lung tissue biomechanics imaged with synchrotron phase contrast microtomography in live rats. Scientific Reports, 2022, 12 (1), pp.5056-1-5056-11. ⟨10.1038/s41598-022-09052-9⟩. ⟨hal-03722030⟩
6 View
4 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More