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An Efficient Chromatin Immunoprecipitation Protocol for the Analysis of Histone Modification Distributions in the Brown Alga Ectocarpus
Abstract : The brown algae are an important but understudied group of multicellular marine organisms. A number of genetic and genomic tools have been developed for the model brown alga Ectocarpus; this includes, most recently, chromatin immunoprecipitation methodology, which allows genome-wide detection and analysis of histone post-translational modifications. Post-translational modifications of histone molecules have been shown to play an important role in gene regulation in organisms from other major eukaryotic lineages, and this methodology will therefore be a very useful tool to investigate genome function in the brown algae. This article provides a detailed, step-by-step description of the Ectocarpus ChIP protocol, which effectively addresses the difficult problem of efficiently extracting chromatin from cells protected by a highly resistant cell wall. The protocol described here will be an essential tool for the future application of chromatin analysis methodologies in brown algal research.
https://hal-cnrs.archives-ouvertes.fr/hal-03658367
Contributor : J. Mark Cock Connect in order to contact the contributor
Submitted on : Tuesday, May 3, 2022 - 6:53:59 PM
Last modification on : Thursday, May 5, 2022 - 3:36:24 AM
Contributor : J. Mark Cock Connect in order to contact the contributor
Submitted on : Tuesday, May 3, 2022 - 6:53:59 PM
Last modification on : Thursday, May 5, 2022 - 3:36:24 AM
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mps-05-00036.pdf
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