Scarless Removal of Large Resistance Island AbaR Results in Antibiotic Susceptibility and Increased Natural Transformability in Acinetobacter baumannii - Archive ouverte HAL Access content directly
Journal Articles Antimicrobial Agents and Chemotherapy Year : 2020

Scarless Removal of Large Resistance Island AbaR Results in Antibiotic Susceptibility and Increased Natural Transformability in Acinetobacter baumannii

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Anne-Sophie Godeux
  • Function : Author
Elin Svedholm
  • Function : Author
Agnese Lupo
  • Function : Author
Marisa Haenni
Xavier Charpentier

Abstract

With a great diversity in gene composition, including multiple putative antibiotic resistance genes, AbaR islands are potential contributors to multidrug resistance in Acinetobacter baumannii . However, the effective contribution of AbaR to antibiotic resistance and bacterial physiology remains elusive. To address this, we sought to accurately remove AbaR islands and restore the integrity of their insertion site. To this end, we devised a versatile scarless genome editing strategy.

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hal-03548950 , version 1 (31-01-2022)

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Anne-Sophie Godeux, Elin Svedholm, Agnese Lupo, Marisa Haenni, Samuel Venner, et al.. Scarless Removal of Large Resistance Island AbaR Results in Antibiotic Susceptibility and Increased Natural Transformability in Acinetobacter baumannii. Antimicrobial Agents and Chemotherapy, 2020, 64 (10), ⟨10.1128/AAC.00951-20⟩. ⟨hal-03548950⟩
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