DNA damage after in vitro exposure to very low concentration of combusted cerium nanoparticles (CoCeO2NPs) and to CoCeO2NPs + Benzo(a)pyrene in rat and human gametes

Melanie Auffan (1) , Martina Cotena , Virginie Tassistro , Antoine Netter , Jérôme Rose , Jeanne Perrin

1 CEREGE - Centre européen de recherche et d'enseignement des géosciences de l'environnement

Titre 25 mots 25 DNA damage after in vitro exposure to very low concentration of combusted cerium nanoparticles (CoCeO 2 NPs) and to CoCeO 2 NPs + Benzo(a)pyrene in rat and human gametes. Study question 25 mots 23 Do in vitro gametes exposures to very low concentration of CoCeO 2 NPs and to CoCeO 2 NPs + Benzo(a)pyrene induce significant and similar DNA damage ? Summary answer 25 mots 22 In vitro gametes co-exposure to CoCeO 2 NPs + Benzo(a)pyrene induced statistically higher DNA damage than exposure to CoCeO 2 NPs alone in rat and human. What is known already 100 mots 100 Cerium dioxide nanoparticles (CeO 2 NPs) are widely used as diesel additive and are released in the air after engine combustion. The Organization for Economic Cooperation and Development included CeO 2 NPs in the priority list of nanomaterials requiring urgent evaluation. In vitro exposure of human and mouse sperm and cumulus oocyte complexes (COC) to very low concentrations of pristine CeO 2 NPs induce significant DNA damage. Benzo(a)pyrene (BaP) is also released in the air by diesel combustion and is known to induce DNA damage in sperm and COC. Nevertheless, pristine CeO 2 NPs are modified by combustion; the impact of CoCeO 2 NPs exposure and to CoCeO 2 NPs+BaP remains unexplored. Study design, size, duration 75mots 66 Pristine CeO 2 NPs were extracted from Envirox TM diesel additive and combusted at 850°C, (average combustion temperature in a diesel engine), to obtain CoCeO 2 NPs. Crystalline structure of CoCeO 2 NPs was analysed by X-ray Diffraction. Rat gametes were sampled in epididymis and oviducts after euthanasia of five mature males and fourty 4 weeks old females (after ovarian stimulation). Human frozen sperm from fertile donors were purchased from Germetheque biobank (France).

Domaines: Sciences de l'environnement

Melanie Auffan, Martina Cotena, Virginie Tassistro, Antoine Netter, Jérôme Rose, et al.. DNA damage after in vitro exposure to very low concentration of combusted cerium nanoparticles (CoCeO2NPs) and to CoCeO2NPs + Benzo(a)pyrene in rat and human gametes. 35th annual meeting of the European Society of Human Reproduction and Embryology, Jun 2019, Vienna, Austria. ⟨hal-03539365⟩