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Slug Is Required for Cell Survival during Partial Epithelial-Mesenchymal Transition of HGF-induced Tubulogenesis

Abstract : Transcription factors of the Snail family are key regulators of epithelial-mesenchymal transition (EMT). In many processes during development or disease, cells do not acquire all the characteristics associated with EMT, leading to what we refer to as partial EMT (p-EMT). However, little is known of the implications of the Snail transcription factors in processes that only involve a p-EMT. To assess this, we used the hepatocyte growth factor (HGF)-induced Madin-Darby canine kidney tubulogenesis system, which provides a three-dimensional culture model of a morphogenetic process including a p-EMT. We found that although Slug (Snail2) is highly and transitory up-regulated during the p-EMT phase of tubulogenesis, it is not a repressor of E-cadherin during this process. Using inducible knockdown of Slug, we demonstrate that Slug is not an inducer of cell movement and instead is required for survival during p-EMT. We conclude that in epithelial cells, promoting cell survival can be a primary function of Slug, rather than being acquired concomitantly with EMT.
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https://hal-cnrs.archives-ouvertes.fr/hal-03490056
Contributor : Pascale Leroy Connect in order to contact the contributor
Submitted on : Friday, December 17, 2021 - 5:55:15 PM
Last modification on : Thursday, March 17, 2022 - 12:20:04 PM

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Pascale Leroy, Keith E Mostov. Slug Is Required for Cell Survival during Partial Epithelial-Mesenchymal Transition of HGF-induced Tubulogenesis. Molecular Biology of the Cell, 2007, 18 (5), pp.1943-1952. ⟨10.1091/mbc.e06-09-0823⟩. ⟨hal-03490056⟩

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