Skip to Main content Skip to Navigation
Journal articles

Novel Insights into Quantitative Proteomics from an Innovative Bottom-Up Simple Light Isotope Metabolic (bSLIM) Labeling Data Processing Strategy

Abstract : Simple light isotope metabolic labeling (SLIM labeling) is an innovative method to quantify variations in the proteome based on an original in vivo labeling strategy. Heterotrophic cells grown in U-[ 12 C] as the sole source of carbon synthesize U-[ 12 C]-amino acids, which are incorporated into proteins, giving rise to U-[ 12 C]-proteins. This results in a large increase in the intensity of the monoisotope ion of peptides and proteins, thus allowing higher identification scores and protein sequence coverage in mass spectrometry experiments. This method, initially developed for signal processing and quantification of the incorporation rate of 12 C into peptides, was based on a multistep process that was difficult to implement for many laboratories. To overcome these limitations, we developed a new theoretical background to analyze bottom-up proteomics data using SLIM-labeling (bSLIM) and established simple procedures based on open-source software, using dedicated OpenMS modules, and embedded R scripts to process the bSLIM experimental data. These new tools allow computation of both the 12 C abundance in peptides to follow the kinetics of protein labeling and the molar fraction of unlabeled and 12 C-labeled peptides in multiplexing experiments to determine the relative abundance of proteins extracted under different biological conditions. They also make it possible to consider incomplete 12 C labeling, such as that observed in cells with nutritional requirements for nonlabeled amino acids. These tools were validated on an experimental dataset produced using various yeast strains of Saccharomyces cerevisiae and growth conditions. The workflows are built on the implementation of appropriate calculation modules in a KNIME working environment. These new integrated tools provide a convenient framework for the wider use of the SLIM-labeling strategy.
Document type :
Journal articles
Complete list of metadata

https://hal-cnrs.archives-ouvertes.fr/hal-03452224
Contributor : Jean-Michel Camadro Connect in order to contact the contributor
Submitted on : Friday, November 26, 2021 - 6:19:59 PM
Last modification on : Tuesday, January 4, 2022 - 6:01:00 AM

File

2021_Sénécaut-N_J.Proteome.R...
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Nicolas Sénécaut, Gelio Alves, Hendrik Weisser, Laurent Lignières, Samuel Terrier, et al.. Novel Insights into Quantitative Proteomics from an Innovative Bottom-Up Simple Light Isotope Metabolic (bSLIM) Labeling Data Processing Strategy. Journal of Proteome Research, American Chemical Society, 2021, 20 (3), pp.1476 - 1487. ⟨10.1021/acs.jproteome.0c00478⟩. ⟨hal-03452224⟩

Share

Metrics

Les métriques sont temporairement indisponibles