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Journal articles
micro-RNA 21 detection with a limit of 2 pM in 1 min using a size-accordable concentration module operated by electrohydrodynamic actuation
Abstract : We present a fluorimetry-based technology for micro-RNA-21 (miR-21) sensing based on the
concentration of miR-molecular beacon (MB) complexes and flushing of unbound MB. This
concentration module consists of a microfluidic channel with the shape of a funnel operated with
electrohydrodynamic actuation. We report a limit of detection of 2 pM in less than one minute for
miR-21 alone, and then demonstrate that miR-21 levels measured in fine needle biopsy samples
from patients with pancreatic cancer correlate with the reference technique of reverse-
transcription polymerase chain reaction (RT-PCR). Altogether, this technology has promising
clinical performances for the follow-up of patients with cancer.
https://hal-cnrs.archives-ouvertes.fr/hal-03449104
Contributor : Aurélien Bancaud Connect in order to contact the contributor
Submitted on : Friday, November 26, 2021 - 2:47:55 PM
Last modification on : Tuesday, April 5, 2022 - 3:44:36 AM
Long-term archiving on: : Sunday, February 27, 2022 - 7:26:23 PM
Contributor : Aurélien Bancaud Connect in order to contact the contributor
Submitted on : Friday, November 26, 2021 - 2:47:55 PM
Last modification on : Tuesday, April 5, 2022 - 3:44:36 AM
Long-term archiving on: : Sunday, February 27, 2022 - 7:26:23 PM
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