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micro-RNA 21 detection with a limit of 2 pM in 1 min using a size-accordable concentration module operated by electrohydrodynamic actuation

Abstract : We present a fluorimetry-based technology for micro-RNA-21 (miR-21) sensing based on the concentration of miR-molecular beacon (MB) complexes and flushing of unbound MB. This concentration module consists of a microfluidic channel with the shape of a funnel operated with electrohydrodynamic actuation. We report a limit of detection of 2 pM in less than 1 min for miR-21 alone, and then demonstrate that miR-21 levels, measured in fine needle biopsy samples, from patients with pancreatic cancer correlate with the reference technique of reverse-transcription polymerase chain reaction (RT-PCR). Altogether, this technology has promising clinical performances for the follow-up of patients with cancer.
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https://hal-cnrs.archives-ouvertes.fr/hal-03444566
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Submitted on : Tuesday, November 23, 2021 - 5:18:01 PM
Last modification on : Tuesday, November 23, 2021 - 5:18:02 PM

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Inga Tijunelyte, Rémi Malbec, Bayan Chami, Jean Cacheux, Christophe Dez, et al.. micro-RNA 21 detection with a limit of 2 pM in 1 min using a size-accordable concentration module operated by electrohydrodynamic actuation. Biosensors and Bioelectronics, Elsevier, 2021, 178, pp.112992. ⟨10.1016/j.bios.2021.112992⟩. ⟨hal-03444566⟩

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