micro-RNA 21 detection with a limit of 2 pM in 1 min using a size-accordable concentration module operated by electrohydrodynamic actuation

Inga Tijunelyte , Rémi Malbec , Bayan Chami , Jean Cacheux , Christophe Dez , Thierry Leichlé (1, 2) , Pierre Cordelier , Aurélien Bancaud

1 Georgia Tech Lorraine [Metz]
2 ECE GeorgiaTech - School of Electrical and Computer Engineering - Georgia Insitute of Technology

We present a fluorimetry-based technology for micro-RNA-21 (miR-21) sensing based on the concentration of miR-molecular beacon (MB) complexes and flushing of unbound MB. This concentration module consists of a microfluidic channel with the shape of a funnel operated with electrohydrodynamic actuation. We report a limit of detection of 2 pM in less than 1 min for miR-21 alone, and then demonstrate that miR-21 levels, measured in fine needle biopsy samples, from patients with pancreatic cancer correlate with the reference technique of reverse-transcription polymerase chain reaction (RT-PCR). Altogether, this technology has promising clinical performances for the follow-up of patients with cancer.

Mots clés: Electrohydrodynamic migration; RNA/DNA concentration; miR-21; Fine needle biopsy

Domaines: Sciences de l'ingénieur [physics]

Inga Tijunelyte, Rémi Malbec, Bayan Chami, Jean Cacheux, Christophe Dez, et al.. micro-RNA 21 detection with a limit of 2 pM in 1 min using a size-accordable concentration module operated by electrohydrodynamic actuation. Biosensors and Bioelectronics, 2021, 178, pp.112992. ⟨10.1016/j.bios.2021.112992⟩. ⟨hal-03444566⟩