Skip to Main content Skip to Navigation
Journal articles

Magnetic Resonance Imaging of Protease-Mediated Lung Tissue Inflammation and Injury

Abstract : Pulmonary inflammation usually involves strong neutrophil recruitment with a marked release of proteases such as neutrophil elastase (NE). Noninvasive in vivo assessment of unregulated elastase activity in the lungs would provide a valuable diagnostic tool. Here, it is proposed to use Overhauser-enhanced magnetic resonance imaging (OMRI) in mice where inflammation was induced by the instillation of lipopolysaccharide (LPS). OMRI contrast in the lungs was generated by a dedicated NE free radical substrate. The free radical decayed more rapidly in LPS-treated mouse lungs than in control mice, indicating the occurrence of increased proteolysis under inflammation. Preclinical detection of abnormal proteolysis opens the way for new diagnosis modality and antiprotease testing in vivo.
Document type :
Journal articles
Complete list of metadata

https://hal-cnrs.archives-ouvertes.fr/hal-03366656
Contributor : Karine Viaud Connect in order to contact the contributor
Submitted on : Tuesday, October 5, 2021 - 5:33:46 PM
Last modification on : Wednesday, October 6, 2021 - 4:48:28 PM

File

Rivot et al. - 2021 - Magnetic...
Publisher files allowed on an open archive

Licence


Distributed under a Creative Commons Attribution - NonCommercial - NoDerivatives 4.0 International License

Identifiers

Collections

Citation

Angélique Rivot, Natacha Jugniot, Samuel Jacoutot, Nicolas Vanthuyne, Philippe Massot, et al.. Magnetic Resonance Imaging of Protease-Mediated Lung Tissue Inflammation and Injury. ACS Omega, ACS Publications, 2021, 6 (23), pp.15012 - 15016. ⟨10.1021/acsomega.1c01150⟩. ⟨hal-03366656⟩

Share

Metrics

Record views

8

Files downloads

3