Magnetic Resonance Imaging of Protease-Mediated Lung Tissue Inflammation and Injury - Archive ouverte HAL Access content directly
Journal Articles ACS Omega Year : 2021

Magnetic Resonance Imaging of Protease-Mediated Lung Tissue Inflammation and Injury

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Sylvain R.A. Marque

Abstract

Pulmonary inflammation usually involves strong neutrophil recruitment with a marked release of proteases such as neutrophil elastase (NE). Noninvasive in vivo assessment of unregulated elastase activity in the lungs would provide a valuable diagnostic tool. Here, it is proposed to use Overhauser-enhanced magnetic resonance imaging (OMRI) in mice where inflammation was induced by the instillation of lipopolysaccharide (LPS). OMRI contrast in the lungs was generated by a dedicated NE free radical substrate. The free radical decayed more rapidly in LPS-treated mouse lungs than in control mice, indicating the occurrence of increased proteolysis under inflammation. Preclinical detection of abnormal proteolysis opens the way for new diagnosis modality and antiprotease testing in vivo.
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Dates and versions

hal-03366656 , version 1 (05-10-2021)

Licence

Attribution - NonCommercial - NoDerivatives - CC BY 4.0

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Angélique Rivot, Natacha Jugniot, Samuel Jacoutot, Nicolas Vanthuyne, Philippe Massot, et al.. Magnetic Resonance Imaging of Protease-Mediated Lung Tissue Inflammation and Injury. ACS Omega, 2021, 6 (23), pp.15012 - 15016. ⟨10.1021/acsomega.1c01150⟩. ⟨hal-03366656⟩
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