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Fluorescent nanocarriers targeting VCAM-1 for early detection of senescent endothelial cells

Abstract : Endothelial senescence has been identified as an early event in the development of endothelial dysfunction, a hallmark of cardiovascular disease. This study developed theranostic nanocarriers (NC) decorated with VCAM-1 antibodies (NC-VCAM-1) in order to target cell surface VCAM-1, which is overexpressed in senescent endothelial cells (ECs) for diagnostic and therapeutic purposes. Incubation of Ang IIinduced premature senescent ECs or replicative senescent ECs with NC-VCAM-1 loaded with lipophilic fluorescent dyes showed higher fluorescence signals than healthy EC, which was dependent on the NC size and VCAM-1 antibodies concentration, and not observed following masking of VCAM-1. NC loaded with omega 3 polyunsaturated fatty acid (NC-EPA:DHA6:1) were more effective than native EPA:DHA 6:1 to prevent Ang II-induced VCAM-1 and p53 upregulation, and SA-β-galactosidase activity in coronary artery segments. These theranostic NC might be of interest to evaluate the extent and localization of endothelial senescence and to prevent pro-senescent endothelial responses.
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https://hal-cnrs.archives-ouvertes.fr/hal-03357935
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Submitted on : Monday, October 11, 2021 - 9:57:54 AM
Last modification on : Tuesday, January 4, 2022 - 5:55:24 AM
Long-term archiving on: : Wednesday, January 12, 2022 - 6:41:21 PM

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Eugenia Belcastro, Asad Rehman, Lamia Remila, Sin-Hee Park, Dal Seong Gong, et al.. Fluorescent nanocarriers targeting VCAM-1 for early detection of senescent endothelial cells. Nanomedicine: Nanotechnology, Biology and Medicine, Elsevier, 2021, 34, ⟨10.1016/j.nano.2021.102379⟩. ⟨hal-03357935⟩

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