Fluorescent nanocarriers targeting VCAM-1 for early detection of senescent endothelial cells - Archive ouverte HAL Access content directly
Journal Articles Nanomedicine: Nanotechnology, Biology and Medicine Year : 2021

Fluorescent nanocarriers targeting VCAM-1 for early detection of senescent endothelial cells

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Abstract

Endothelial senescence has been identified as an early event in the development of endothelial dysfunction, a hallmark of cardiovascular disease. This study developed theranostic nanocarriers (NC) decorated with VCAM-1 antibodies (NC-VCAM-1) in order to target cell surface VCAM-1, which is overexpressed in senescent endothelial cells (ECs) for diagnostic and therapeutic purposes. Incubation of Ang IIinduced premature senescent ECs or replicative senescent ECs with NC-VCAM-1 loaded with lipophilic fluorescent dyes showed higher fluorescence signals than healthy EC, which was dependent on the NC size and VCAM-1 antibodies concentration, and not observed following masking of VCAM-1. NC loaded with omega 3 polyunsaturated fatty acid (NC-EPA:DHA6:1) were more effective than native EPA:DHA 6:1 to prevent Ang II-induced VCAM-1 and p53 upregulation, and SA-β-galactosidase activity in coronary artery segments. These theranostic NC might be of interest to evaluate the extent and localization of endothelial senescence and to prevent pro-senescent endothelial responses.
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Dates and versions

hal-03357935 , version 1 (11-10-2021)

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Eugenia Belcastro, Asad Ur Rehman, Lamia Remila, Sin-Hee Park, Dal Seong Gong, et al.. Fluorescent nanocarriers targeting VCAM-1 for early detection of senescent endothelial cells. Nanomedicine: Nanotechnology, Biology and Medicine, 2021, 34, ⟨10.1016/j.nano.2021.102379⟩. ⟨hal-03357935⟩
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