Targeted CRISPR‐Cas9‐based gene knockouts in the model brown alga Ectocarpus - Archive ouverte HAL Access content directly
Journal Articles New Phytologist Year : 2021

Targeted CRISPR‐Cas9‐based gene knockouts in the model brown alga Ectocarpus

(1, 2) , (1, 2) , (3) , (1) , (1) , (1) , (4, 5) , (1, 2) , (6) , (6) , (1, 2)
1
2
3
4
5
6

Abstract

Brown algae are an important group of multicellular eukaryotes, phylogenetically distinct from both the animal and land plant lineages. Ectocarpus has emerged as a model organism to study diverse aspects of brown algal biology, but this system currently lacks an effective reverse genetics methodology to analyse the functions of selected target genes. Here, we report that mutations at specific target sites are generated following the introduction of CRISPR-Cas9 ribonucleoproteins into Ectocarpus cells, using either biolistics or microinjection as the delivery method. Individuals with mutations affecting the ADENINE PHOSPHORIBOSYL TRANSFERASE (APT) gene were isolated following treatment with 2-fluoroadenine, and this selection system was used to isolate individuals in which mutations had been introduced simultaneously at APT and at a second gene. This double mutation approach could potentially be used to isolate mutants affecting any Ectocarpus gene, providing an effective reverse genetics tool for this model organism. The availability of this tool will significantly enhance the utility of Ectocarpus as a model organism for this ecologically and economically important group of marine organisms. Moreover, the methodology described here should be readily transferable to other brown algal species.
Fichier principal
Vignette du fichier
Ectocarpus_CRISPR_paper_25may21.pdf (2.11 Mo) Télécharger le fichier
Origin : Files produced by the author(s)

Dates and versions

hal-03356727 , version 1 (28-09-2021)

Identifiers

Cite

Yacine Badis, Delphine Scornet, Minori Harada, Céline Caillard, Olivier Godfroy, et al.. Targeted CRISPR‐Cas9‐based gene knockouts in the model brown alga Ectocarpus. New Phytologist, 2021, 231 (5), pp.2077-2091. ⟨10.1111/nph.17525⟩. ⟨hal-03356727⟩
52 View
153 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More