Structure of the ubiquitous 3' processing enzyme RNase Z bound to transfer RNA

Inès Li de La Sierra-Gallay (1, 2) , Nathalie Mathy (3) , Olivier Pellegrini (3) , Ciarán Condon (3)

1 I2BC - Institut de Biologie Intégrative de la Cellule
2 IBPC (FR_550) - Institut de biologie physico-chimique
3 REGCM - Régulation de l'expression génétique chez les microorganismes - Expression génétique microbienne

The highly conserved ribonuclease RNase Z catalyzes the endonucleolytic removal of the 3' extension of the majority of tRNA precursors. Here we present the structure of the complex between Bacillus subtilis RNase Z and tRNA(Thr), the first structure of a ribonucleolytic processing enzyme bound to tRNA. Binding of tRNA to RNase Z causes conformational changes in both partners to promote reorganization of the catalytic site and tRNA cleavage

Domaines: Sciences du Vivant [q-bio]; Biochimie, Biologie Moléculaire; Biologie structurale [q-bio.BM]

Inès Li de La Sierra-Gallay, Nathalie Mathy, Olivier Pellegrini, Ciarán Condon. Structure of the ubiquitous 3' processing enzyme RNase Z bound to transfer RNA. Nature Structural and Molecular Biology, 2006, 13 (4), pp.376-377. ⟨10.1038/nsmb1066⟩. ⟨hal-03347756⟩