The 5S rRNA maturase, ribonuclease M5, is a Toprim domain family member

Frédéric Allemand (1) , Nathalie Mathy (2) , Dominque Brechemier-Baey (2) , Ciarán Condon (2)

1 CBS - Centre de Biochimie Structurale [Montpellier]
2 REGCM - Régulation de l'expression génétique chez les microorganismes

The maturation of 5S ribosomal RNA in low G+C Gram-positive bacteria is catalyzed by a highly conserved, ∼190 residue, enzyme, called ribonuclease M5 (RNase M5). Sequence alignment had predicted that the N-terminal half of RNase M5 would consist of a Toprim domain, a protein fold found in type IA and type II topoisomerases, DnaG-like primases, OLD family nucleases and RecR proteins [L. Aravind, D. D. Leipe and E. V. Koonin (1998) Nucleic Acids Res., 26, 4205–4213]. Here, we present structural modelling data and a mutational analysis of RNase M5 that confirms this hypothesis. The N-terminal half of RNase M5 can be fitted to the Toprim domain of the DnaG catalytic core. Mutation of amino acid residues highly conserved among RNase M5 enzymes and members of the Toprim domain family showed that alteration of residues critical for topoisomerase and primase activity also had a dramatic effect on the cleavage of 5S rRNA precursor by RNase M5 both in vivo and in vitro. This suggests that the mechanisms of double-stranded RNA cleavage by RNase M5 and double-stranded DNA cleavage by members of the topoisomerase family are related.

Domaines: Génomique, Transcriptomique et Protéomique [q-bio.GN]

Frédéric Allemand, Nathalie Mathy, Dominque Brechemier-Baey, Ciarán Condon. The 5S rRNA maturase, ribonuclease M5, is a Toprim domain family member. Nucleic Acids Research, 2005, 33 (13), pp.4368-4376. ⟨10.1093/nar/gki752⟩. ⟨hal-03347730⟩