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Freeze-dried fecal samples are biologically active after long-lasting storage and suited to fecal microbiota transplantation in a preclinical murine model ofClostridioides difficileinfection

Abstract : Fecal microbiota transplantation is now recommended for treating recurrent forms ofClostridioides difficileinfection. Recent studies have reported protocols using capsules of either frozen or freeze-dried stool allowing oral administration in in- and out-patient settings. However, a central question remains the viability, engraftment, and efficacy of the microbiome over time during storage life. This study shows that both the freeze-drying and freezing procedures for fecal samples allowed preserving viability, short-chain fatty acids concentration, and anti-Clostridioides difficileproperties of microbiota without significant alteration after storage for 12 months. Fecal transplantation with freeze-dried microbiota allowed engraftment of microbiota leading to clearance ofClostridioides difficileinfection in a preclinical murine model with a survival rate of 70%versus53-60% in mice treated with frozen inocula, and 20% in the untreated group. Moreover, the freeze-dried powder can be used to fill oral hard capsules using a very low amount (0.5%) of glidant excipient, allowing oral formulation. Altogether, this study showed that freeze-dried inocula can be used for the treatment ofClostridioides difficileinfection with long-lasting stability of the fecal microbiota. This formulation facilitates biobanking and allows the use of hard capsules, an essential step to simplify patient access to treatment.
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https://hal-cnrs.archives-ouvertes.fr/hal-03292691
Contributor : Colette Orange <>
Submitted on : Tuesday, July 20, 2021 - 3:22:54 PM
Last modification on : Friday, July 23, 2021 - 3:53:00 AM

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Julie Reygner, Christine Charrueau, Johanne Delannoy, Camille Mayeur, Veronique Robert, et al.. Freeze-dried fecal samples are biologically active after long-lasting storage and suited to fecal microbiota transplantation in a preclinical murine model ofClostridioides difficileinfection. Gut microbes, Taylor & Francis, 2020, 11 (5), pp.1405-1422. ⟨10.1080/19490976.2020.1759489⟩. ⟨hal-03292691⟩

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