Lipoplexes Strengthened by Anionic Polymers: Easy Preparation of Highly Effective siRNA Vectors Based on Cationic Lipids and Anionic Polymers - Archive ouverte HAL Access content directly
Journal Articles Methods in molecular biology (Clifton, N.J.) Year : 2016

Lipoplexes Strengthened by Anionic Polymers: Easy Preparation of Highly Effective siRNA Vectors Based on Cationic Lipids and Anionic Polymers

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Abstract

RNA interference is an invaluable tool in biology to specifically silence a given gene. Synthetic duplexes of RNA oligonucleotides are widely used to induce mRNA degradation in cultured cells or in whole organisms. They have to be vectorized to reach their target site. Here, we describe the preparation of highly efficient siRNA vectors based on cationic liposomes and polyanionic polymers and their application in cultured cells to silence reporter and/or endogenous genes.
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hal-03292064 , version 1 (20-07-2021)

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Danielle Campiol Arruda, Anne Schlegel, Pascal Bigey, Virginie Escriou. Lipoplexes Strengthened by Anionic Polymers: Easy Preparation of Highly Effective siRNA Vectors Based on Cationic Lipids and Anionic Polymers. Methods in molecular biology (Clifton, N.J.), 2016, 1445, pp.137-48. ⟨10.1007/978-1-4939-3718-9_8⟩. ⟨hal-03292064⟩
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