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Electrochemical DNA-biosensors based on long-range electron transfer: optimization of the amperometric detection in the femtomolar range using two-electrode setup and ultramicroelectrode

Abstract : We report on the design of electrochemical DNA-biosensors made from 15, 25 and 50 mm diameter gold ultramicroelectrodes (UMEs) in a two-electrode setup. The biosensors were prepared by chemical adsorption of 22-base thiol-labeled DNA-probes in a self-assembled monolayer configuration onto the surface of the gold UME. Hybridization events were transduced by Fe-III/Fe-II redox process electromediated by DNA-redox intercalated mediators. The optimization of the DNA-biosensors was achieved by using different FeIII/FeII ligands (cyanide, cefadroxil, deferoxamine) and intercalators (methylene blue, proflavine). Optimized biosensors allowed direct and selective amperometric detection in the femtomolar range and of single mismatch within a 35 minutes protocol. (C) 2016 Elsevier Ltd. All rights reserved.
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https://hal-cnrs.archives-ouvertes.fr/hal-03292058
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Submitted on : Tuesday, July 20, 2021 - 10:30:02 AM
Last modification on : Wednesday, December 15, 2021 - 10:03:29 AM

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A. Nassi, F-X. Guillon, A. Amar, B. Hainque, S. Amriche, et al.. Electrochemical DNA-biosensors based on long-range electron transfer: optimization of the amperometric detection in the femtomolar range using two-electrode setup and ultramicroelectrode. Electrochimica Acta, Elsevier, 2016, 209, pp.269-277. ⟨10.1016/j.electacta.2016.04.144⟩. ⟨hal-03292058⟩

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