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HAL-CNRSCNRS scientific publications |
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Journal articles
Engineering of PEDF-Expressing Primary Pigment Epithelial Cells by the SB Transposon System Delivered by pFAR4 Plasmids
Abstract : Neovascular age-related macular degeneration (nvAMD) is characterized by choroidal blood vessels growing into the sub-retinal space, leading to retinal pigment epithelial (RPE) cell degeneration and vision loss. Vessel growth results from an imbalance of pro-angiogenic (e.g., vascular endothelial growth factor [VEGF]) and anti-angiogenic factors (e.g., pigment epithelium-derived factor [PEDF]). Current treatment using intravitreal injections of anti-VEGF antibodies improves vision in about 30% of patients but may be accompanied by side effects and non-compliance. To avoid the difficulties posed by frequent intravitreal injections, we have proposed the transplantation of pigment epithelial cells modified to overexpress human PEDF. Stable transgene integration and expression is ensured by the hyperactive Sleeping Beauty transposon system delivered by pFAR4 miniplasmids, which have a backbone free of antibiotic resistance markers. We demonstrated efficient expression of the PEDF gene and an optimized PEDF cDNA sequence in as few as 5 x 10(3) primary cells. At 3 weeks post-transfection, PEDF secretion was significantly elevated and long-term follow-up indicated a more stable secretion by cells transfected with the optimized PEDF transgene. Analysis of transgene insertion sites in human RPE cells showed an almost random genomic distribution. The results represent an important contribution toward a clinical trial aiming at a non-viral gene therapy of nvAMD.
https://hal-cnrs.archives-ouvertes.fr/hal-03292053
Contributor : Colette Orange Connect in order to contact the contributor
Submitted on : Friday, September 10, 2021 - 10:54:48 AM
Last modification on : Friday, January 7, 2022 - 3:53:56 AM
Long-term archiving on: : Saturday, December 11, 2021 - 6:33:52 PM
Contributor : Colette Orange Connect in order to contact the contributor
Submitted on : Friday, September 10, 2021 - 10:54:48 AM
Last modification on : Friday, January 7, 2022 - 3:53:56 AM
Long-term archiving on: : Saturday, December 11, 2021 - 6:33:52 PM