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Journal Articles Anticancer Research Year : 2016

Metabolism of Flavone-8-acetic Acid in Mice

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Minh Hien Pham
  • Function : Author
Nicolas Auzeil
  • Function : Author
  • PersonId : 766416
  • IdRef : 067255329
Anne Regazzetti
  • Function : Author
Daniel Scherman
  • Function : Author
Daniel Dauzonne
  • Function : Author

Abstract

Flavone-8-acetic acid (FAA) is a potent antivascular agent in mice but not in humans. Assuming that FAA was bioactivated in mice, we previously demonstrated that 6-OH-FAA was formed from FAA by mouse microsomes but not by human microsomes; its antivascular activity was 2.1- to 15.9-fold stronger than that of FAA, and its antivascular activity was mediated through the Ras homolog gene family (Rho) protein kinase A (RhoA) pathway. The present work aimed to study FAA metabolism in order to verify if 6-OH-FAA is formed in mice. Using synthesized standards and high-performance liquid chromatography (HPLC) coupled with ultraviolet (UV) detection and mass spectrometry (MS) analysis, we herein demonstrated, for the first time, that in vitro FAA and its monohydroxylated derivatives could directly undergo phase II metabolism forming glucuronides, and two FAA epoxides were mostly scavenged by NAC and GSH forming corresponding adducts. FAA was metabolized in mice. Several metabolites were formed, in particular 6-OHFAA. The antitumor activity of 6-OH-FAA in vivo is worthy of investigation.
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Dates and versions

hal-03290548 , version 1 (19-07-2021)

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  • HAL Id : hal-03290548 , version 1

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Minh Hien Pham, Nicolas Auzeil, Anne Regazzetti, Daniel Scherman, Johanne Seguin, et al.. Metabolism of Flavone-8-acetic Acid in Mice. Anticancer Research, 2016, 36 (8), pp.3889-3898. ⟨hal-03290548⟩
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