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Metabolism of Flavone-8-acetic Acid in Mice

Abstract : Flavone-8-acetic acid (FAA) is a potent antivascular agent in mice but not in humans. Assuming that FAA was bioactivated in mice, we previously demonstrated that 6-OH-FAA was formed from FAA by mouse microsomes but not by human microsomes; its antivascular activity was 2.1- to 15.9-fold stronger than that of FAA, and its antivascular activity was mediated through the Ras homolog gene family (Rho) protein kinase A (RhoA) pathway. The present work aimed to study FAA metabolism in order to verify if 6-OH-FAA is formed in mice. Using synthesized standards and high-performance liquid chromatography (HPLC) coupled with ultraviolet (UV) detection and mass spectrometry (MS) analysis, we herein demonstrated, for the first time, that in vitro FAA and its monohydroxylated derivatives could directly undergo phase II metabolism forming glucuronides, and two FAA epoxides were mostly scavenged by NAC and GSH forming corresponding adducts. FAA was metabolized in mice. Several metabolites were formed, in particular 6-OHFAA. The antitumor activity of 6-OH-FAA in vivo is worthy of investigation.
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https://hal-cnrs.archives-ouvertes.fr/hal-03290548
Contributor : Colette Orange <>
Submitted on : Monday, July 19, 2021 - 1:40:25 PM
Last modification on : Friday, July 23, 2021 - 3:53:01 AM

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  • HAL Id : hal-03290548, version 1

Citation

Minh Hien Pham, Nicolas Auzeil, Anne Regazzetti, Daniel Scherman, Johanne Seguin, et al.. Metabolism of Flavone-8-acetic Acid in Mice. Anticancer Research, International Institute of Anticancer Research, 2016, 36 (8), pp.3889-3898. ⟨hal-03290548⟩

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