The arachidonic acid metabolite 11β-ProstaglandinF2α controls intestinal epithelial healing: deficiency in patients with Crohn's disease - Archive ouverte HAL Access content directly
Journal Articles Scientific Reports Year : 2016

The arachidonic acid metabolite 11β-ProstaglandinF2α controls intestinal epithelial healing: deficiency in patients with Crohn's disease

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Sabrina Coquenlorge
  • Function : Author
Laurianne van Landeghem
Julie Jaulin
  • Function : Author
Nicolas Cenac
Nathalie Vergnolle
  • Function : Author
Emilie Duchalais
  • Function : Author
Michel Neunlist

Abstract

In healthy gut enteric glial cells (EGC) are essential to intestinal epithelial barrier (IEB) functions. In Crohn's Disease (CD), both EGC phenotype and IEB functions are altered, but putative involvement of EGC in CD pathogenesis remains unknown and study of human EGC are lacking. EGC isolated from CD and control patients showed similar expression of glial markers and EGC-derived soluble factors (IL6, TGF-β, proEGF, GSH) but CD EGC failed to increase IEB resistance and healing. Lipid profiling showed that CD EGC produced decreased amounts of 15-HETE, 18-HEPE, 15dPGJ 2 and 11βPGF 2 α as compared to healthy EGC. They also had reduced expression of the L-PGDS and AKR1C3 enzymes. Produced by healthy EGC, the 11βPGF 2 activated PPARγ receptor of intestinal epithelial cells to induce cell spreading and IEB wound repair. In addition to this novel healing mechanism our data show that CD EGC presented impaired ability to promote IEB functions through defect in L-PGDS-AKR1C3-11βPGF 2 α dependent pathway.
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hal-03153609 , version 1 (26-02-2021)

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Sabrina Coquenlorge, Laurianne van Landeghem, Julie Jaulin, Nicolas Cenac, Nathalie Vergnolle, et al.. The arachidonic acid metabolite 11β-ProstaglandinF2α controls intestinal epithelial healing: deficiency in patients with Crohn's disease. Scientific Reports, 2016, 6 (1), ⟨10.1038/srep25203⟩. ⟨hal-03153609⟩

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