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The arachidonic acid metabolite 11β-ProstaglandinF2α controls intestinal epithelial healing: deficiency in patients with Crohn's disease

Abstract : In healthy gut enteric glial cells (EGC) are essential to intestinal epithelial barrier (IEB) functions. In Crohn's Disease (CD), both EGC phenotype and IEB functions are altered, but putative involvement of EGC in CD pathogenesis remains unknown and study of human EGC are lacking. EGC isolated from CD and control patients showed similar expression of glial markers and EGC-derived soluble factors (IL6, TGF-β, proEGF, GSH) but CD EGC failed to increase IEB resistance and healing. Lipid profiling showed that CD EGC produced decreased amounts of 15-HETE, 18-HEPE, 15dPGJ 2 and 11βPGF 2 α as compared to healthy EGC. They also had reduced expression of the L-PGDS and AKR1C3 enzymes. Produced by healthy EGC, the 11βPGF 2 activated PPARγ receptor of intestinal epithelial cells to induce cell spreading and IEB wound repair. In addition to this novel healing mechanism our data show that CD EGC presented impaired ability to promote IEB functions through defect in L-PGDS-AKR1C3-11βPGF 2 α dependent pathway.
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https://hal-cnrs.archives-ouvertes.fr/hal-03153609
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Submitted on : Friday, February 26, 2021 - 3:16:59 PM
Last modification on : Tuesday, March 2, 2021 - 12:40:51 PM
Long-term archiving on: : Thursday, May 27, 2021 - 6:48:11 PM

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Sabrina Coquenlorge, Laurianne van Landeghem, Julie Jaulin, Nicolas Cenac, Nathalie Vergnolle, et al.. The arachidonic acid metabolite 11β-ProstaglandinF2α controls intestinal epithelial healing: deficiency in patients with Crohn's disease. Scientific Reports, Nature Publishing Group, 2016, 6 (1), ⟨10.1038/srep25203⟩. ⟨hal-03153609⟩

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