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The viral F-box protein P0 induces an ER-derived autophagy degradation pathway for the clearance of membrane-bound AGO1
Abstract : RNA silencing is a major antiviral defense mechanism in plants and
invertebrates. Plant ARGONAUTE1 (AGO1) is pivotal in RNA silencing, and hence is a major target for counteracting viral suppressors
of RNA-silencing proteins (VSRs). P0 from Turnip yellows virus
(TuYV) is a VSR that was previously shown to trigger AGO1 degradation via an autophagy-like process. However, the identity of
host proteins involved and the cellular site at which AGO1 and P0
interact were unknown. Here we report that P0 and AGO1 associate on the endoplasmic reticulum (ER), resulting in their loading
into ER-associated vesicles that are mobilized to the vacuole in an
ATG5- and ATG7-dependent manner. We further identified ATG8-
Interacting proteins 1 and 2 (ATI1 and ATI2) as proteins that associate with P0 and interact with AGO1 on the ER up to the vacuole.
Notably, ATI1 and ATI2 belong to an endogenous degradation
pathway of ER-associated AGO1 that is significantly induced following P0 expression. Accordingly, ATI1 and ATI2 deficiency causes
a significant increase in posttranscriptional gene silencing (PTGS)
activity. Collectively, we identify ATI1 and ATI2 as components of
an ER-associated AGO1 turnover and proper PTGS maintenance and
further show how the VSR P0 manipulates this pathway.
https://hal-cnrs.archives-ouvertes.fr/hal-03096615
Contributor : Veronique Ziegler-Graff Connect in order to contact the contributor
Submitted on : Tuesday, January 5, 2021 - 9:19:52 AM
Last modification on : Monday, October 11, 2021 - 1:22:26 PM
Long-term archiving on: : Wednesday, April 7, 2021 - 9:30:03 AM
Contributor : Veronique Ziegler-Graff Connect in order to contact the contributor
Submitted on : Tuesday, January 5, 2021 - 9:19:52 AM
Last modification on : Monday, October 11, 2021 - 1:22:26 PM
Long-term archiving on: : Wednesday, April 7, 2021 - 9:30:03 AM
File
Michaeli et al. PNAS 2019.pdf
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