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High Pathogenicity of Nipah Virus from Pteropus lylei Fruit Bats, Cambodia

Abstract : We conducted an in-depth characterization of the Nipah virus (NiV) isolate previously obtained from a Pteropus lylei bat in Cambodia in 2003 (CSUR381). We performed full-genome sequencing and phylogenetic analyses and confirmed CSUR381 is part of the NiV-Malaysia genotype. In vitro studies revealed similar cell permissiveness and replication of CSUR381 (compared with 2 other NiV isolates) in both bat and human cell lines. Sequence alignments indicated conservation of the ephrin-B2 and ephrin-B3 receptor binding sites, the glycosylation site on the G attachment protein, as well as the editing site in phosphoprotein, suggesting production of nonstructural proteins V and W, known to counteract the host innate immunity. In the hamster animal model, CSUR381 induced lethal infections. Altogether, these data suggest that the Cambodia bat-derived NiV isolate has high pathogenic potential and, thus, provide insight for further studies and better risk assessment for future NiV outbreaks in Southeast Asia.
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https://hal-cnrs.archives-ouvertes.fr/hal-03091393
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Submitted on : Thursday, December 31, 2020 - 12:17:18 AM
Last modification on : Tuesday, October 19, 2021 - 3:07:21 PM
Long-term archiving on: : Thursday, April 1, 2021 - 6:07:16 PM

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Maria Gaudino, Noémie Aurine, Claire Dumont, Julien Fouret, Marion Ferren, et al.. High Pathogenicity of Nipah Virus from Pteropus lylei Fruit Bats, Cambodia. Emerging Infectious Diseases, Centers for Disease Control and Prevention, 2020, 26 (1), pp.104 - 113. ⟨10.3201/eid2601.191284⟩. ⟨hal-03091393⟩

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