Characterization of the S locus genes, SLG and SRK, of the Brassica S3 haplotype: identification of a membrane-localized protein encoded by the S locus receptor kinase gene

Valerie Delorme (1) , Jean-Loic Giranton (1) , Yves Hatzfeld (1, 2) , Aline Friry (1, 2) , Philippe Heizmann (1, 2) , Maria Jose Ariza (1, 2) , Christian Dumas (1, 2) , Thierry Gaude (1, 2) , J. Mark Cock (1)

1 RDP - Reproduction et développement des plantes
2 Laboratoire associé de Reconnaissance cellulaire et amélioration des plantes

The S locus, which controls the self-incompatibility response in Brassica, has been shown to contain at least two genes. SLG encodes a secreted S locus glycoprotein whilst SRK encodes a putative S locus receptor kinase. SRK has been shown potentially to encode a functional kinase and genetic evidence indicates that this gene is essential for the self-incompatibility response. Here the characterization of the SRK and SLG genes of a Brassice line homozygous for the S3 haplotype is described. A 120 kDa glycoprotein was identified in stigmas and several lines of evidence indicated that this protein is encoded by the SRK 3 gene. First, the 120 kDa glycoprotein was recognized by antibodies raised against peptides based on the SRK 3 gene sequence. Secondly, this protein is polymorphic and, in an F 2 population segregating for the S3 haplotype, was expressed only in plants possessing the S3 haplotype. Thirdly, the 120 kDa protein was expressed specifically in stigmas. Finally, the 120 kDa protein was only extracted from stigmas in the presence of detergent indicating that it is anchored in the membrane. SRK has been predicted to encode a transmembrane glycoprotein based on the deduced amino acid sequence. Located on the membrane, SRK is in a position to interface between an extracellular recognition event between pollen and pistil and an intracellular signal transduction pathway which initiates the self-incompatibility response.

Domains: Life Sciences [q-bio]

Valerie Delorme, Jean-Loic Giranton, Yves Hatzfeld, Aline Friry, Philippe Heizmann, et al.. Characterization of the S locus genes, SLG and SRK, of the Brassica S3 haplotype: identification of a membrane-localized protein encoded by the S locus receptor kinase gene. Plant Journal, 1995, 7 (3), pp.429-440. ⟨10.1046/j.1365-313x.1995.7030429.x⟩. ⟨hal-03086441⟩