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Efficient transformation of Medicago truncatula cv. Jemalong using the hypervirulent Agrobacterium tumefaciens strain AGL1

Abstract : The efficiency of Agrobacterium tumefaciens transformation of the model legume Medicago truncatula cv. Jemalong (genotype 2HA) was evaluated for strains LBA 4404, C58pMP90, C58pGV2260 and AGL1. Binary vectors carrying promoter-gus/gfp reporter gene fusions and the nptII gene as selectable marker were used for plant in vitro transformation/regeneration. The highest transformation efficiency was obtained with the disarmed hypervirulent strain AGL1 (Ti plasmid TiBo542), for which the percentage of explants forming kanamycin (Km)-resistant calli was double that obtained with each of the other three strains. In addition, we were able to reduce the time necessary for plant regeneration using AGL1, with 24% of the explants generating Km-resistant transgenic plantlets within only 4-5 months of culture. Transgene expression in planta was analysed and found to be conserved in the T-1 descendents.
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https://hal-cnrs.archives-ouvertes.fr/hal-03082480
Contributor : Fernanda de Carvalho-Niebel <>
Submitted on : Friday, December 18, 2020 - 3:56:19 PM
Last modification on : Wednesday, May 12, 2021 - 8:16:58 AM

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M. Chabaud, Fernanda de Carvalho-Niebel, D. Barker. Efficient transformation of Medicago truncatula cv. Jemalong using the hypervirulent Agrobacterium tumefaciens strain AGL1. Plant Cell Reports, Springer Verlag, 2003, 22 (1), pp.46-51. ⟨10.1007/s00299-003-0649-y⟩. ⟨hal-03082480⟩

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