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Transcript enrichment of Nod factor-elicited early nodulin genes in purified root hair fractions of the model legume Medicago truncatula

Abstract : This article describes an efficient procedure to study Nod factor-induced gene expression in root hairs of the model legume Medicago truncatula. By developing an improved method of fracturing frozen root hairs, it has been possible to obtain a highly purified root hair fraction from M. truncatula seedlings yielding sufficient RNA for real-time quantitative RT-PCR expression analysis. After Nod factor treatment it was possible to detect up to 100-fold increases of MtENOD11 and pMtENOD11-gus transcript levels in root hair RNA. This corresponds to 5-7-fold higher induction levels than for entire root tissue preparations. Furthermore, the use of these enriched RNA samples has revealed for the first time a very significant induction (30-fold) of the MtENOD40 gene in root hairs in response to Nod factors. It is concluded that the rapid and convenient procedure described here will be particularly useful for detecting tissue-specific low-level gene expression in root hairs responding to Rhizobium Nod factors or other exogenous signals.
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https://hal-cnrs.archives-ouvertes.fr/hal-03082465
Contributor : Fernanda de Carvalho-Niebel Connect in order to contact the contributor
Submitted on : Friday, December 18, 2020 - 3:54:08 PM
Last modification on : Wednesday, September 22, 2021 - 1:46:04 PM

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Laurent Sauviac, Andréas Niebel, Aurélien Boisson-Dernier, David Barker, Fernanda de Carvalho-Niebel. Transcript enrichment of Nod factor-elicited early nodulin genes in purified root hair fractions of the model legume Medicago truncatula. Journal of Experimental Botany, Oxford University Press (OUP), 2005, 56 (419), pp.2507-2513. ⟨10.1093/jxb/eri244⟩. ⟨hal-03082465⟩

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