Transcript enrichment of Nod factor-elicited early nodulin genes in purified root hair fractions of the model legume Medicago truncatula - Archive ouverte HAL Access content directly
Journal Articles Journal of Experimental Botany Year : 2005

Transcript enrichment of Nod factor-elicited early nodulin genes in purified root hair fractions of the model legume Medicago truncatula

(1) , (1) , (1) , (1) , (1)
1

Abstract

This article describes an efficient procedure to study Nod factor-induced gene expression in root hairs of the model legume Medicago truncatula. By developing an improved method of fracturing frozen root hairs, it has been possible to obtain a highly purified root hair fraction from M. truncatula seedlings yielding sufficient RNA for real-time quantitative RT-PCR expression analysis. After Nod factor treatment it was possible to detect up to 100-fold increases of MtENOD11 and pMtENOD11-gus transcript levels in root hair RNA. This corresponds to 5-7-fold higher induction levels than for entire root tissue preparations. Furthermore, the use of these enriched RNA samples has revealed for the first time a very significant induction (30-fold) of the MtENOD40 gene in root hairs in response to Nod factors. It is concluded that the rapid and convenient procedure described here will be particularly useful for detecting tissue-specific low-level gene expression in root hairs responding to Rhizobium Nod factors or other exogenous signals.

Dates and versions

hal-03082465 , version 1 (18-12-2020)

Identifiers

Cite

Laurent Sauviac, Andréas Niebel, Aurélien Boisson-Dernier, David Barker, Fernanda de Carvalho-Niebel. Transcript enrichment of Nod factor-elicited early nodulin genes in purified root hair fractions of the model legume Medicago truncatula. Journal of Experimental Botany, 2005, 56 (419), pp.2507-2513. ⟨10.1093/jxb/eri244⟩. ⟨hal-03082465⟩
32 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More