Production, in Pichia pastoris, of a recombinant monomeric mapacalcine, a protein with anti-ischemic properties - Archive ouverte HAL Access content directly
Journal Articles Biochemistry and Biophysics Reports Year : 2015

Production, in Pichia pastoris, of a recombinant monomeric mapacalcine, a protein with anti-ischemic properties

, (1) , (2) , , , , (2) , , (3) , (2) , (2)
1
2
3

Abstract

Mapacalcine is a small homodimeric protein of 19 kDa with 9 disulfide bridges extracted from the Cliona vastifica sponge (Red Sea). It selectively blocks a calcium current insensitive to most calcium blockers. Specific receptors for mapacalcine have been described in a variety of tissues such as brain, smooth muscle, liver, and kidney. Previous works achieved on hepatocytes and nervous cells demonstrated that this protein selectively blocks a calcium influx triggered by an ischemia/reperfusion (I/R) shock and efficiently protects cells from death after I/R. The aim of this work was to produce the recombinant mapacalcine in the yeast Pichia pastoris. Mass spectrometry, light scattering analysis and biological characterization demonstrated that the recombinant mapacalcine obtained was a monomeric form with 4 disulfide bridges which retains the biological activity of the natural protein.
Fichier principal
Vignette du fichier
2015-NoubhaniAetal.pdf (1.81 Mo) Télécharger le fichier
Origin : Publication funded by an institution

Dates and versions

hal-03048086 , version 1 (09-12-2020)

Identifiers

Cite

A. Noubhani, Dominique Begu, S. Chaignepain, H. Moha Ou Maati, M. Borsotto, et al.. Production, in Pichia pastoris, of a recombinant monomeric mapacalcine, a protein with anti-ischemic properties. Biochemistry and Biophysics Reports, 2015, 4, pp.299 - 305. ⟨10.1016/j.bbrep.2015.10.003⟩. ⟨hal-03048086⟩
33 View
32 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More