Red wine extract disrupts Th17 lymphocyte differentiation in a colorectal cancer context

Pauline Chalons (1) , Flavie Courtaut (1) , Emeric Limagne (1) , Fanny F Chalmin (1) , Emma Cantos-Villar (2) , Tristan Richard (3) , Cyril Auger (4) , Philippe Chabert (5) , Valérie Schini- Kerth (6) , François Ghiringhelli (1) , Virginie Aires (1) , Dominique Delmas (1)

1 LNC - Lipides - Nutrition - Cancer [Dijon - U1231]
2 IFAPA - Instituto Andaluz de Investigación y Formación Agraria y Pesquera
3 OENO - Unité de Recherche Oenologie [Villenave d'Ornon]
4 NanoRegMed - Nanomédecine Régénérative
5 LBP - Laboratoire de Bioimagerie et Pathologies
6 C2VN - Centre recherche en CardioVasculaire et Nutrition = Center for CardioVascular and Nutrition research

Scope: Scope: It is well established that immune response and inflammation promote tumoral progression. Immune cells communicate through direct contact or through cytokine secretion, and it is the pro-inflammatory status that will tip the balance toward tumor progression or anti-tumor immunity. It is demonstrated here that a red wine extract (RWE) can decrease inflammation through its action on the inflammasome complex. This study determines whether an RWE could impact other key actors of inflammation, including T helper 17 (Th17) immune cells in particular. Methods and results: Methods and results: Using an RWE containing 4.16 g of polyphenols/liter of wine, it is shown that RWE decreases colorectal cancer cells in vitro and induces a reduction in colorectal tumor growth associated with a decrease in tumor-infiltrating lymphocytes in vivo. The process of T-lymphocyte differentiation in Th17 cells is altered by RWE, as revealed by the decrease in the expression of key actors controlling this process, such as signal transducer and activator of transcription 3 and retinoid acid-related orphan receptor t. This disruption is associated with an inhibition of inflammatory interleukin 17 secretion. Conclusion: The data highlights the major involvement of Th17 immune cells in the biological effects of an RWE.

Keywords: Polyphenols; lymphocytes Th17; lymphocyte T Red wine extract; chemoprevention; colon cancer; red wine extract; Cancers polyphenols; Dominique; Cancers Delmas; Virginie; Cancers Lipids; Fanny; Plateforme de Transfert en Biologie du Cancer (PTBC) Chalmin; Cancers; Emeric; Cancers Limagne; Flavie; Cancers Courtaut; Nutrition; Lipids; Interleukins

Domains: Life Sciences [q-bio]; Human health and pathology

Pauline Chalons, Flavie Courtaut, Emeric Limagne, Fanny F Chalmin, Emma Cantos-Villar, et al.. Red wine extract disrupts Th17 lymphocyte differentiation in a colorectal cancer context. Molecular Nutrition and Food Research, 2020, 64 (11), pp.1901286. ⟨10.1002/mnfr.201901286⟩. ⟨hal-03033795⟩