Oral Intake of EPA:DHA 6:1 by Middle-Aged Rats for One Week Improves Age-Related Endothelial Dysfunction in Both the Femoral Artery and Vein: Role of Cyclooxygenases - Archive ouverte HAL Access content directly
Journal Articles International Journal of Molecular Sciences Year : 2020

Oral Intake of EPA:DHA 6:1 by Middle-Aged Rats for One Week Improves Age-Related Endothelial Dysfunction in Both the Femoral Artery and Vein: Role of Cyclooxygenases

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Sébastien Gaertner
  • Function : Author
  • PersonId : 1083776
Cyril Auger
Muhammad A Farooq
  • Function : Author
  • PersonId : 1083777
Brigitte Pollet
  • Function : Author
  • PersonId : 1083778
Sophie Schrevens
  • Function : Author
  • PersonId : 1083779
Florence Toti
  • Function : Author
  • PersonId : 1076639
Valérie B Schini-Kerth
  • Function : Author
  • PersonId : 1055270

Abstract

In humans, aging is associated with endothelial dysfunction and an increased risk of venous thromboembolism. Although intake of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) at a ratio of 6:1 by old rats improved the endothelial dysfunction in arteries, the impact on veins remains unclear. Eight-month-old male Wistar rats were either untreated or orally administered corn oil, EPA:DHA 1:1, or EPA:DHA 6:1 (500 mg/kg/d) for seven days. Vascular reactivity was studied by myography. In middle-aged femoral artery rings, acetylcholine caused a partial relaxation at low concentrations and a contractile response at high concentrations, whereas in the old femoral vein only a partial relaxation was observed. The EPA:DHA 6:1 treatment blunted the contractile response to acetylcholine in the middle-aged femoral artery and both EPA:DHA 6:1 and 1:1 increased the relaxation to acetylcholine in the old femoral vein. No such effects were observed with corn oil. Both the non-selective cyclooxygenase inhibitor indomethacin and the COX-1 inhibitor SC-560 increased the relaxation to acetylcholine in the middle-aged femoral artery whereas the COX-2 inhibitor NS-398 increased that in the middle-aged femoral vein. In conclusion, our results indicate that aging is associated with an endothelial dysfunction in the femoral artery and vein, which can be improved by EPA:DHA 6:1 treatment-most likely via a cyclooxygenase-dependent mechanism.
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Dates and versions

hal-03033691 , version 1 (01-12-2020)

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Sébastien Gaertner, Cyril Auger, Muhammad A Farooq, Brigitte Pollet, Sonia Khemais-Benkhiat, et al.. Oral Intake of EPA:DHA 6:1 by Middle-Aged Rats for One Week Improves Age-Related Endothelial Dysfunction in Both the Femoral Artery and Vein: Role of Cyclooxygenases. International Journal of Molecular Sciences, 2020, 21, pp.113749. ⟨10.3390/ijms21030920⟩. ⟨hal-03033691⟩
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