Organization of the albumin and alpha-fetoprotein genes in fetal and adult rat tissues, and rat hepatomas

Abstract : We compared the organization of the albumin and alpha-fetoprotein (AFP) genes in chromosomal DNA from different fetal and adult rat tissues as well as from two rat hepatomas. These two genes are expressed at widely different levels in the tissues and hepatomas analysed. Southern blots of DNAs digested with the restriction endonucleases EcoRI, HindIII or MspI were hybridized to albumin and AFP complementary DNA (cDNA) and genomic probes. No significant difference was observed in the hybridization patterns obtained for the DNAs from the different tissues, except for some interstrain variation between the chromosomal DNAs isolated from Sprague-Dawley and Buffalo rats, which was due to allelic polymorphism. We cannot rule out the possibility of changes in chromosomal gene organization which would result either in small alterations of restriction fragment size or in translocations of large blocks of DNA containing whole sets of restriction enzyme fragments within the chromosome; however, our results indicate that the gross organization of the albumin and AFP genes remains constant throughout the regulatory processes involved in the tissue- and time-specific transcription of these genes.