An alternative spliced form of FosB is a negative regulator of transcriptional activation and transformation by Fos proteins. - Archive ouverte HAL Access content directly
Journal Articles Proceedings of the National Academy of Sciences of the United States of America Year : 1991

An alternative spliced form of FosB is a negative regulator of transcriptional activation and transformation by Fos proteins.

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1
J. Yen
  • Function : Author
R. Wisdom
  • Function : Author
I. Tratner
I. Verma
  • Function : Author

Abstract

Two forms of FosB transcript and their products can be identified in mouse NIH 3T3 cells following serum induction. The larger RNA codes for a 338-amino acid protein, whereas the smaller RNA results from the removal of an additional 140 nucleotides from FosB mRNA by alternative splicing. This alternative splicing event places a stop codon following the "leucine zipper" region and results in a shorter protein (FosB2) of 237 amino acids that lacks 101 amino acids at the carboxyl terminus. FosB2 is able to form heterodimers with c-Jun and bind to an AP-1 site but is not able to activate the transcription of promoters containing AP-1 sites. Furthermore, FosB2 can not only suppress the transcriptional activation by c-Fos and c-Jun of promoters containing an AP-1 site but also interferes with the transforming potential of viral and cellular Fos proteins. We propose that FosB2 protein functions as a trans-negative regulator.

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hal-03028954 , version 1 (27-11-2020)

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J. Yen, R. Wisdom, I. Tratner, I. Verma. An alternative spliced form of FosB is a negative regulator of transcriptional activation and transformation by Fos proteins.. Proceedings of the National Academy of Sciences of the United States of America, 1991, 88 (12), pp.5077-5081. ⟨10.1073/pnas.88.12.5077⟩. ⟨hal-03028954⟩

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