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CRISPR-mediated biocontainment

Abstract : We have exploited the repetitive nature of transposable elements of the human genome to generate synthetic circuits. Transposable elements such as LINE-1 and Alu have successfully replicated in mammalian genomes throughout evolution to reach a copy number ranging from thousands to more than a million. Targeting these repetitive elements with programmable DNA nucleases such as CRISPR-Cas9 rapidly induce extremely high levels of cell death. We use this genotoxic feature to build synthetic biocontainment circuits: CRISPR defense system (CRISPR-DS) capable of preventing CRISPR genome editing, and we introduce the proof-of-concept of CRISPR Safety-Switch, an inducible, stringent and non-leaky kill-switch capable of clearing out cell lines resistant to DNA breaks. .
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https://hal-cnrs.archives-ouvertes.fr/hal-03027524
Contributor : Hannu Myllykallio Connect in order to contact the contributor
Submitted on : Friday, November 27, 2020 - 10:56:04 AM
Last modification on : Wednesday, December 2, 2020 - 3:37:57 AM
Long-term archiving on: : Sunday, February 28, 2021 - 6:58:53 PM

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2020.02.03.922146v1.full.pdf
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Oscar Castanon, Cory Smith, Parastoo Khoshakhlagh, Raphael Ferreira, Marc Güell, et al.. CRISPR-mediated biocontainment. 2020. ⟨hal-03027524⟩

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