HAL will be down for maintenance from Friday, June 10 at 4pm through Monday, June 13 at 9am. More information
Skip to Main content Skip to Navigation
Journal articles

Link between steroidogenesis, the cell cycle, and PKA in adrenocortical tumor cells

Abstract : Adrenocortical tumors (ACTs) frequently cause steroid excess and present cell-cycle dysregulation. cAMP/PKA signaling is involved in steroid synthesis and play a role in cell-cycle regulation. We investigated, by cell synchronization in the different phases of the cell-cycle, the control of steroidogenesis and the contribution of PKA in adrenocortical cells (H295R and culture of primary pigmented nodular adrenocortical disease cells). Cells showed increased steroidogenesis and a maximal PKA activity at G2 phase, and a reduction at G1 phase. PRKACA overexpression, or cAMP stimulation, enhanced PKA activity and induced steroidogenesis in all synchronized groups but is not sufficient to drive cell-cycle progression. PRKAR1A inactivation enhanced PKA activity and induced STAR gene expression, only in cells in G1, and triggered cell-cycle progression in all groups. These findings provide evidence for a tight association between steroidogenesis and cell-cycle in ACTs. Moreover, PRKAR1A is essential for mediating the function of PKA activity on both steroidogenesis and cell-cycle progression in adrenocortical cells.
Document type :
Journal articles
Complete list of metadata

Cited literature [40 references]  Display  Hide  Download

https://hal-cnrs.archives-ouvertes.fr/hal-03014038
Contributor : Marthe Rizk-Rabin Connect in order to contact the contributor
Submitted on : Thursday, November 19, 2020 - 11:37:35 AM
Last modification on : Thursday, April 7, 2022 - 1:58:18 PM
Long-term archiving on: : Saturday, February 20, 2021 - 6:59:50 PM

File

Molecular and cellular Endocri...
Files produced by the author(s)

Identifiers

Collections

Citation

Marthe Rizk-Rabin, Sabrina Chaoui-Ibadioune, Anna Vaczlavik, Christopher Ribes, Michel Polak, et al.. Link between steroidogenesis, the cell cycle, and PKA in adrenocortical tumor cells. Molecular and Cellular Endocrinology, Elsevier, 2020, 500, ⟨10.1016/j.mce.2019.110636⟩. ⟨hal-03014038⟩

Share

Metrics

Record views

36

Files downloads

93