Crystal Structure of the Arcelin-1 Dimer from Phaseolus vulgaris at 1.9-Å Resolution - Archive ouverte HAL Access content directly
Journal Articles Journal of Biological Chemistry Year : 1998

Crystal Structure of the Arcelin-1 Dimer from Phaseolus vulgaris at 1.9-Å Resolution

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Abstract

Arcelin-1 is a glycoprotein from kidney beans (Phaseo-lus vulgaris) which displays insecticidal properties and protects the seeds from predation by larvae of various bruchids. This lectin-like protein is devoid of monosac-charide binding properties and belongs to the phytohe-magglutinin protein family. The x-ray structure determination at 1.9-Å resolution of native arcelin-1 dimers, which correspond to the functional state of the protein in solution, was solved using multiple isomorphous replacement and refined to a crystallographic R factor of 0.208. The three glycosylation sites on each monomer are all covalently modified. One of these oligosaccharide chains provides interactions with protein atoms at the dimer interface, and another one may act by preventing the formation of higher oligomeric species in the arcelin variants. The dimeric structure and the severe alteration of the monosaccharide binding site in arcelin-1 correlate with the hemagglutinating properties of the protein, which are unaffected by simple sugars and sugar derivatives. Sequence analysis and structure comparisons of arcelin-1 with the other insecticidal proteins from kidney beans, arcelin-5, and ␣-amylase inhibitor and with legume lectins, yield insights into the molecular basis of the different biological functions of these proteins.
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hal-03004563 , version 1 (13-11-2020)

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Lionel Mourey, Jean-Denis Pedelacq, Catherine Birck, Christine Fabre, Pierre Rougé, et al.. Crystal Structure of the Arcelin-1 Dimer from Phaseolus vulgaris at 1.9-Å Resolution. Journal of Biological Chemistry, 1998, 273 (21), pp.12914-12922. ⟨10.1074/jbc.273.21.12914⟩. ⟨hal-03004563⟩
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