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Crystal Structure of the Arcelin-1 Dimer from Phaseolus vulgaris at 1.9-Å Resolution
Abstract : Arcelin-1 is a glycoprotein from kidney beans (Phaseo-lus vulgaris) which displays insecticidal properties and protects the seeds from predation by larvae of various bruchids. This lectin-like protein is devoid of monosac-charide binding properties and belongs to the phytohe-magglutinin protein family. The x-ray structure determination at 1.9-Å resolution of native arcelin-1 dimers, which correspond to the functional state of the protein in solution, was solved using multiple isomorphous replacement and refined to a crystallographic R factor of 0.208. The three glycosylation sites on each monomer are all covalently modified. One of these oligosaccharide chains provides interactions with protein atoms at the dimer interface, and another one may act by preventing the formation of higher oligomeric species in the arcelin variants. The dimeric structure and the severe alteration of the monosaccharide binding site in arcelin-1 correlate with the hemagglutinating properties of the protein, which are unaffected by simple sugars and sugar derivatives. Sequence analysis and structure comparisons of arcelin-1 with the other insecticidal proteins from kidney beans, arcelin-5, and ␣-amylase inhibitor and with legume lectins, yield insights into the molecular basis of the different biological functions of these proteins.
Cited literature [59 references]
https://hal-cnrs.archives-ouvertes.fr/hal-03004563
Contributor : Lionel Mourey Connect in order to contact the contributor
Submitted on : Friday, November 13, 2020 - 4:36:15 PM
Last modification on : Thursday, November 25, 2021 - 5:22:04 PM
Long-term archiving on: : Sunday, February 14, 2021 - 7:39:52 PM
Contributor : Lionel Mourey Connect in order to contact the contributor
Submitted on : Friday, November 13, 2020 - 4:36:15 PM
Last modification on : Thursday, November 25, 2021 - 5:22:04 PM
Long-term archiving on: : Sunday, February 14, 2021 - 7:39:52 PM
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mourey-jbiolchem98.pdf
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