W. C. Moore, A. T. Hastie, X. Li, H. Li, W. W. Busse et al., Sputum neutrophil counts are associated with more severe asthma phenotypes using cluster analysis, Journal of Allergy and Clinical Immunology, vol.133, issue.6, pp.1557-1563.e5, 2014.

N. Krishnamoorthy, D. N. Douda, T. R. Brüggemann, I. Ricklefs, M. G. Duvall et al., Neutrophil cytoplasts induce TH17 differentiation and skew inflammation toward neutrophilia in severe asthma, Science Immunology, vol.3, issue.26, p.eaao4747, 2018.

T. K. Wright, P. G. Gibson, J. L. Simpson, V. M. Mcdonald, L. G. Wood et al., Neutrophil extracellular traps are associated with inflammation in chronic airway disease, Respirology, vol.21, issue.3, pp.467-475, 2016.

R. Y. Kim, J. W. Pinkerton, A. T. Essilfie, A. A. Robertson, K. J. Baines et al., Role for NLRP3 Inflammasome?mediated, IL-1??Dependent Responses in Severe, Steroid-Resistant Asthma, American Journal of Respiratory and Critical Care Medicine, vol.196, issue.3, pp.283-297, 2017.

C. Rossios, S. Pavlidis, U. Hoda, C. Kuo, C. Wiegman et al., Sputum transcriptomics reveal upregulation of IL-1 receptor family members in patients with severe asthma, Journal of Allergy and Clinical Immunology, vol.141, issue.2, pp.560-570, 2018.

V. Delgado-rizo, M. A. Martínez-guzmán, L. Iñiguez-gutierrez, A. García-orozco, A. Alvarado-navarro et al., Neutrophil Extracellular Traps and Its Implications in Inflammation: An Overview, Frontiers in Immunology, vol.8, p.81, 2017.

K. Schroder and J. Tschopp, The Inflammasomes, Cell, vol.140, issue.6, pp.821-832, 2010.

Y. Zhang, S. Saccani, H. Shin, and B. S. Nikolajczyk, Dynamic Protein Associations Define Two Phases of IL-1? Transcriptional Activation, The Journal of Immunology, vol.181, issue.1, pp.503-512, 2008.

G. Sollberger, G. E. Strittmatter, M. Garstkiewicz, J. Sand, and H. Beer, Caspase-1: The inflammasome and beyond, Innate Immunity, vol.20, issue.2, pp.115-125, 2013.

S. Kerr, High Sputum DNA Concentrations in a Subset of Asthmatics with Neutrophilic Inflammation Is Associated with More Severe Morbidity, C33. NOVEL BIOMARKERS IN LUNG DISEASE, pp.5239-5239

W. G. Teague, B. R. Phillips, J. V. Fahy, S. E. Wenzel, A. M. Fitzpatrick et al., Baseline Features of the Severe Asthma Research Program (SARP III) Cohort: Differences with Age, The Journal of Allergy and Clinical Immunology: In Practice, vol.6, issue.2, pp.545-554.e4, 2018.

K. F. Chung, S. E. Wenzel, and J. L. Brozek, Comment on: International ERS/ATS guidelines on definition, evaluation and treatment of severe asthma, European Respiratory Journal, vol.44, issue.1, pp.267-268, 2014.

A. T. Society, Chronic bronchitis, asthma and pulmonary emphysema: a statement by the committee on diagnostic standards for non-tuberculous respiratory diseases, American Review of Respiratory Disease, vol.85, pp.762-768, 1962.

N. H. Gershman, H. H. Wong, J. T. Liu, M. J. Mahlmeister, and J. V. Fahy, Comparison of two methods of collecting induced sputum in asthmatic subjects, European Respiratory Journal, vol.9, issue.12, pp.2448-2453, 1996.

A. T. Hastie, W. C. Moore, H. Li, B. M. Rector, V. E. Ortega et al., Biomarker surrogates do not accurately predict sputum eosinophil and neutrophil percentages in asthmatic subjects, Journal of Allergy and Clinical Immunology, vol.132, issue.1, pp.72-80.e12, 2013.

M. C. Peters, S. Kerr, E. M. Dunican, P. G. Woodruff, M. L. Fajt et al., Refractory airway type 2 inflammation in a large subgroup of asthmatic patients treated with inhaled corticosteroids, Journal of Allergy and Clinical Immunology, vol.143, issue.1, pp.104-113.e14, 2019.

E. Lefrançais, B. Mallavia, H. Zhuo, C. S. Calfee, and M. R. Looney, Maladaptive role of neutrophil extracellular traps in pathogen-induced lung injury, JCI Insight, vol.3, issue.3, p.98178, 2018.

M. O'brien, D. Moehring, R. Muñoz-planillo, G. Núñez, J. Callaway et al., A bioluminescent caspase-1 activity assay rapidly monitors inflammasome activation in cells, Journal of Immunological Methods, vol.447, pp.1-13, 2017.

V. Brinkmann, B. Laube, U. Abu-abed, C. Goosmann, and A. Zychlinsky, Neutrophil Extracellular Traps: How to Generate and Visualize Them, Journal of Visualized Experiments, issue.36, p.2010, 2010.

E. D. Gordon, Alternative splicing of interleukin-33 and type 2 inflammation in asthma, Proc Natl Acad Sci, vol.113, issue.31, pp.8765-70, 2016.

M. E. Lachowicz-scroggins, Cadherin-26 (CDH26) regulates airway epithelial cell cytoskeletal structure and polarity, Cell Discov, vol.4, issue.7, 2018.

H. W. Chu, C. Rios, C. Huang, A. Wesolowska-andersen, E. G. Burchard et al., CRISPR?Cas9-mediated gene knockout in primary human airway epithelial cells reveals a proinflammatory role for MUC18, Gene Therapy, vol.22, issue.10, pp.822-829, 2015.

R. H. Batchelor and M. Zhou, Use of cellular glucose-6-phosphate dehydrogenase for cell quantitation: applications in cytotoxicity and apoptosis assays, Analytical Biochemistry, vol.329, issue.1, pp.35-42, 2004.

G. M. Dolganov, A Novel Method of Gene Transcript Profiling in Airway Biopsy Homogenates Reveals Increased Expression of a Na+-K+-Cl- Cotransporter (NKCC1) in Asthmatic Subjects, Genome Research, vol.11, issue.9, pp.1473-1483, 2001.

P. G. Woodruff, Genome-wide profiling identifies epithelial cell genes associated with asthma and with treatment response to corticosteroids, Proc Natl Acad Sci, vol.104, issue.40, pp.15858-63, 2007.

S. A. Gl-horowitz and . Boyd, Defining, establishing, and verifying reference intervals in the clinical laboratory: approved guideline, 2008.

P. G. Woodruff, R. Khashayar, S. C. Lazarus, S. Janson, P. Avila et al., Relationship between airway inflammation, hyperresponsiveness, and obstruction in asthma, Journal of Allergy and Clinical Immunology, vol.108, issue.5, pp.753-758, 2001.

M. C. Peters, Z. K. Mekonnen, S. Yuan, N. R. Bhakta, P. G. Woodruff et al., Measures of gene expression in sputum cells can identify TH2-high and TH2-low subtypes of asthma, Journal of Allergy and Clinical Immunology, vol.133, issue.2, pp.388-394.e5, 2014.

A. A. Khan, M. A. Alsahli, and A. H. Rahmani, Myeloperoxidase as an Active Disease Biomarker: Its Recent Biochemical and Pathological Perspectives, Med Sci (Basel), issue.6, 2018.

M. O'brien, D. Moehring, R. Muñoz-planillo, G. Núñez, J. Callaway et al., A bioluminescent caspase-1 activity assay rapidly monitors inflammasome activation in cells, Journal of Immunological Methods, vol.447, pp.1-13, 2017.

H. Qi, S. Yang, and L. Zhang, Neutrophil Extracellular Traps and Endothelial Dysfunction in Atherosclerosis and Thrombosis, Frontiers in Immunology, vol.8, p.928, 2017.

M. J. Kaplan and M. Radic, Neutrophil Extracellular Traps: Double-Edged Swords of Innate Immunity, The Journal of Immunology, vol.189, issue.6, pp.2689-2695, 2012.

D. L. Pham, G. Ban, S. Kim, Y. S. Shin, Y. Ye et al., Neutrophil autophagy and extracellular DNA traps contribute to airway inflammation in severe asthma, Clinical & Experimental Allergy, vol.47, issue.1, pp.57-70, 2016.

Y. Choi, L. D. Pham, D. Lee, G. Ban, J. Lee et al., Neutrophil Extracellular DNA Traps Induce Autoantigen Production by Airway Epithelial Cells, Mediators of Inflammation, vol.2017, pp.1-7, 2017.

S. V. Kumar, O. P. Kulkarni, S. R. Mulay, M. N. Darisipudi, S. Romoli et al., Neutrophil Extracellular Trap-Related Extracellular Histones Cause Vascular Necrosis in Severe GN, Journal of the American Society of Nephrology, vol.26, issue.10, pp.2399-2413, 2015.

M. Saffarzadeh, C. Juenemann, M. A. Queisser, G. Lochnit, G. Barreto et al., Neutrophil Extracellular Traps Directly Induce Epithelial and Endothelial Cell Death: A Predominant Role of Histones, PLoS ONE, vol.7, issue.2, p.e32366, 2012.

J. L. Simpson, S. Phipps, K. J. Baines, K. M. Oreo, L. Gunawardhana et al., Elevated expression of the NLRP3 inflammasome in neutrophilic asthma, European Respiratory Journal, vol.43, issue.4, pp.1067-1076, 2013.

F. Awad, E. Assrawi, C. Jumeau, S. Georgin-lavialle, L. Cobret et al., Impact of human monocyte and macrophage polarization on NLR expression and NLRP3 inflammasome activation, PLOS ONE, vol.12, issue.4, p.e0175336, 2017.
URL : https://hal.archives-ouvertes.fr/hal-01509838

G. Sollberger, A. Choidas, G. L. Burn, P. Habenberger, R. Di-lucrezia et al., Gasdermin D plays a vital role in the generation of neutrophil extracellular traps, Science Immunology, vol.3, issue.26, p.eaar6689, 2018.

K. W. Chen, M. Monteleone, D. Boucher, G. Sollberger, D. Ramnath et al., Noncanonical inflammasome signaling elicits gasdermin D?dependent neutrophil extracellular traps, Science Immunology, vol.3, issue.26, p.eaar6676, 2018.

S. Yousefi, C. Mihalache, E. Kozlowski, I. Schmid, and H. U. Simon, Viable neutrophils release mitochondrial DNA to form neutrophil extracellular traps, Cell Death & Differentiation, vol.16, issue.11, pp.1438-1444, 2009.

S. Yousefi, J. A. Gold, N. Andina, J. J. Lee, A. M. Kelly et al., Catapult-like release of mitochondrial DNA by eosinophils contributes to antibacterial defense, Nature Medicine, vol.14, issue.9, pp.949-953, 2008.

Y. Wang, M. Li, S. Stadler, S. Correll, P. Li et al., Histone hypercitrullination mediates chromatin decondensation and neutrophil extracellular trap formation, Journal of Cell Biology, vol.184, issue.2, pp.205-213, 2009.

J. E. Vince and J. Silke, The intersection of cell death and inflammasome activation, Cellular and Molecular Life Sciences, vol.73, issue.11-12, pp.2349-2367, 2016.

M. D. Fahy, National Heart, Lung, and Blood Institute, 2020.