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Alpha technology: A powerful tool to detect mouse brain intracellular signaling events

Marion Zanese Giovanni Tomaselli Valérie Roullot-Lacarrière Maïté Moreau Luigi Bellocchio Agnès Grel Giovanni Marsicano Nathalie Sans Monique Vallée Jean-Michel Revest 1
1 INSERM, Neurocentre Magendie, U1215, Physiopathologie de la Plasticité Neuronale, F-33000 Bordeaux, France;
Abstract : Phosphorylation by protein kinases is a fundamental molecular process involved in the regulation of signaling activities in living organisms. Understanding this complex network of phosphorylation, especially phosphoproteins, is a necessary step for grasping the basis of cellular pathophysiology. Studying brain intracellular signaling is a particularly complex task due to the heterogeneous complex nature of the brain tissue, which consists of many embedded structures.
Keywords : Alpha (Amplified Luminescent Proximity Homogeneous Assay) technology AlphaScreen / AlphaLISA SureFire Ultra Brain Cell signaling Kinases Phosphoproteins
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https://hal-cnrs.archives-ouvertes.fr/hal-02990027
Contributor : Jean-Michel Revest <>
Submitted on : Thursday, November 5, 2020 - 1:46:06 PM
Last modification on : Wednesday, December 2, 2020 - 3:28:35 AM

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Marion Zanese, Giovanni Tomaselli, Valérie Roullot-Lacarrière, Maïté Moreau, Luigi Bellocchio, et al.. Alpha technology: A powerful tool to detect mouse brain intracellular signaling events. Journal of Neuroscience Methods, Elsevier, 2020, 332, pp.108543. ⟨10.1016/j.jneumeth.2019.108543⟩. ⟨hal-02990027⟩

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